Transmembrane protein 173 inhibits RANKL-induced osteoclast differentiation

Chung Hyeon Choe, In Sun Park, Jisang Park, Kang Yeol Yu, Hyonseok Jang, Ju Kim, Yong Suk Jang

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Abstract Tmem173 was identified as a growth inhibitor associated with major histocompatibility complex (MHC) class II and a potential stimulator for IFN-β, an innate immune inducer and a negative feedback controller for RANKL-induced osteoclast differentiation of monocytic macrophage cells. In this study, we confirmed that transmembrane protein 173 (Tmem173) overexpression inhibited the expression of osteoclast-specific genes, tartrate-resistant acid phosphatase (TRAP), cathepsin K, and matrix metalloproteinase-9 (MMP-9), as well as bone resorption pit formation in RANKL-treated RAW 264.7 cells. Activation of osteoclast-specific transcription factors, c-Fos and nuclear factor of activated T cells cytoplasmic-1 (NFATc1), and RANKL-induced activation of ERK were also down-regulated by Tmem173 overexpression. Collectively, these results suggest that Tmem173 plays a regulatory role in RANKL-RANK-mediated signaling in osteoclastogenesis.

    Original languageEnglish
    Article number37040
    Pages (from-to)836-841
    Number of pages6
    JournalFEBS Letters
    Volume589
    Issue number7
    DOIs
    Publication statusPublished - 2015 Mar 24

    Keywords

    • Osteoclastogenesis
    • RANK ligand
    • Tartrate-resistant acid phosphatase
    • Transmembrane protein 173

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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