Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell

Uiyoung Han, Jun Ha Hwang, Jong Min Lee, Hyeoni Kim, Han Sung Jung, Jeong Ho Hong, Jinkee Hong

Research output: Contribution to journalArticle

Abstract

A nanoscale artificial extracellular matrix (nanoshell) formed by layer-by-layer adsorption can enhance and modulate the function of stem cells by transferring biochemical stimulus to the cell directly. Here, the nanoshell composed of fibronectin (FN) and chondroitin sulfate (CS) is demonstrated to promote chondrogenic differentiation of mesenchymal stem cells (MSCs). The multilayer structure of nanoshell is formed by repeating self-assembly of FN and CS, and its thickness can be controlled through the number of layers. The expression of chondrogenic markers in MSCs coated with the FN/CS nanoshell was increased as the number of bilayers in the nanoshell increased until four, but when it exceeds five bilayers, the effect began to decrease. Finally, the MSCs coated with optimized four bilayers of FN/CS nanoshell have high chondrogenic differentiation efficiency and showed the potential to increase formation of cartilage tissue when it is transplanted into mouse kidney. So, the precise regulation of stem cell fate at single cell level can be possible through the cellular surface modification by self-assembled polymeric film.

Original languageEnglish
Pages (from-to)184-193
Number of pages10
JournalBiotechnology and Bioengineering
Volume117
Issue number1
DOIs
Publication statusPublished - 2020 Jan 1

Fingerprint

Nanoshells
Cell membranes
Stem cells
Mesenchymal Stromal Cells
Chondroitin Sulfates
Cell Membrane
Fibronectins
Stem Cells
Cartilage
Polymer films
Self assembly
Adsorption
Extracellular Matrix
Surface treatment
Multilayers
Tissue
Kidney
Sulfates

Keywords

  • chondrogenic differentiation
  • extracellular matrix
  • mesenchymal stem cell
  • polymeric nanofilm

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell. / Han, Uiyoung; Hwang, Jun Ha; Lee, Jong Min; Kim, Hyeoni; Jung, Han Sung; Hong, Jeong Ho; Hong, Jinkee.

In: Biotechnology and Bioengineering, Vol. 117, No. 1, 01.01.2020, p. 184-193.

Research output: Contribution to journalArticle

@article{2d6e8e3c556a4c5db887c7959a49c537,
title = "Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell",
abstract = "A nanoscale artificial extracellular matrix (nanoshell) formed by layer-by-layer adsorption can enhance and modulate the function of stem cells by transferring biochemical stimulus to the cell directly. Here, the nanoshell composed of fibronectin (FN) and chondroitin sulfate (CS) is demonstrated to promote chondrogenic differentiation of mesenchymal stem cells (MSCs). The multilayer structure of nanoshell is formed by repeating self-assembly of FN and CS, and its thickness can be controlled through the number of layers. The expression of chondrogenic markers in MSCs coated with the FN/CS nanoshell was increased as the number of bilayers in the nanoshell increased until four, but when it exceeds five bilayers, the effect began to decrease. Finally, the MSCs coated with optimized four bilayers of FN/CS nanoshell have high chondrogenic differentiation efficiency and showed the potential to increase formation of cartilage tissue when it is transplanted into mouse kidney. So, the precise regulation of stem cell fate at single cell level can be possible through the cellular surface modification by self-assembled polymeric film.",
keywords = "chondrogenic differentiation, extracellular matrix, mesenchymal stem cell, polymeric nanofilm",
author = "Uiyoung Han and Hwang, {Jun Ha} and Lee, {Jong Min} and Hyeoni Kim and Jung, {Han Sung} and Hong, {Jeong Ho} and Jinkee Hong",
year = "2020",
month = "1",
day = "1",
doi = "10.1002/bit.27183",
language = "English",
volume = "117",
pages = "184--193",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "1",

}

TY - JOUR

T1 - Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell

AU - Han, Uiyoung

AU - Hwang, Jun Ha

AU - Lee, Jong Min

AU - Kim, Hyeoni

AU - Jung, Han Sung

AU - Hong, Jeong Ho

AU - Hong, Jinkee

PY - 2020/1/1

Y1 - 2020/1/1

N2 - A nanoscale artificial extracellular matrix (nanoshell) formed by layer-by-layer adsorption can enhance and modulate the function of stem cells by transferring biochemical stimulus to the cell directly. Here, the nanoshell composed of fibronectin (FN) and chondroitin sulfate (CS) is demonstrated to promote chondrogenic differentiation of mesenchymal stem cells (MSCs). The multilayer structure of nanoshell is formed by repeating self-assembly of FN and CS, and its thickness can be controlled through the number of layers. The expression of chondrogenic markers in MSCs coated with the FN/CS nanoshell was increased as the number of bilayers in the nanoshell increased until four, but when it exceeds five bilayers, the effect began to decrease. Finally, the MSCs coated with optimized four bilayers of FN/CS nanoshell have high chondrogenic differentiation efficiency and showed the potential to increase formation of cartilage tissue when it is transplanted into mouse kidney. So, the precise regulation of stem cell fate at single cell level can be possible through the cellular surface modification by self-assembled polymeric film.

AB - A nanoscale artificial extracellular matrix (nanoshell) formed by layer-by-layer adsorption can enhance and modulate the function of stem cells by transferring biochemical stimulus to the cell directly. Here, the nanoshell composed of fibronectin (FN) and chondroitin sulfate (CS) is demonstrated to promote chondrogenic differentiation of mesenchymal stem cells (MSCs). The multilayer structure of nanoshell is formed by repeating self-assembly of FN and CS, and its thickness can be controlled through the number of layers. The expression of chondrogenic markers in MSCs coated with the FN/CS nanoshell was increased as the number of bilayers in the nanoshell increased until four, but when it exceeds five bilayers, the effect began to decrease. Finally, the MSCs coated with optimized four bilayers of FN/CS nanoshell have high chondrogenic differentiation efficiency and showed the potential to increase formation of cartilage tissue when it is transplanted into mouse kidney. So, the precise regulation of stem cell fate at single cell level can be possible through the cellular surface modification by self-assembled polymeric film.

KW - chondrogenic differentiation

KW - extracellular matrix

KW - mesenchymal stem cell

KW - polymeric nanofilm

UR - http://www.scopus.com/inward/record.url?scp=85073977172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073977172&partnerID=8YFLogxK

U2 - 10.1002/bit.27183

DO - 10.1002/bit.27183

M3 - Article

C2 - 31560128

AN - SCOPUS:85073977172

VL - 117

SP - 184

EP - 193

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 1

ER -