Transplantation of adipose derived stem cells for peripheral nerve regeneration in sciatic nerve defects of the rat

Ja Hea Gu, Yi Hwa Ji, Eun-Sang Dhong, Dong Hwee Kim, Eul Sik Yoon

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16 Citations (Scopus)

Abstract

Tissue engineering approaches for promoting the repair of peripheral nerve injuries have focused on cell-based therapies involving Adipose-derived stem cells (ASCs). The authors evaluated the effects of undifferentiated ASCs and of neurally differentiated ASCs on the regenerating abilities of peripheral nerves. We hope that this would demonstrate the feasibility of using adipose derived stem cells for peripheral nerve regeneration and provide clues regarding the use of adipose- derived stem cells. ASCs were isolated and cultured. Then the cells were cultured with neuronal induction agents for neural differentiation. ASCs and neurally differentiated ASCs were transplanted into sciatic nerve defects. After 12 weeks, the number and diameter of the myelinated fibers were measured and nerve conduction study was done. The extent of regeneration of myelinated fibers in the neurally differentiated ASCs transplanted group was greater than that in the ASCs transplanted group or the control group. However, thickness of myelin sheath and diameter of nerve fibers in the ASCs transplanted group were greater than those in the neutrally differentiated ASCs transplanted group or the control group. Nerve conduction study showed good recovery in the neurally differentiated ASCs transplanted groups. Muscles can atrophy and contract if denervation has started. It would be difficult to recover muscle function even if the nerve was reinnervated. Therefore, although neurally differentiated ASCs were found to have a greater functional effect than non-differentiated ASCs, time constraint is important when considering a method of ASCs transplantation.

Original languageEnglish
Pages (from-to)347-355
Number of pages9
JournalCurrent Stem Cell Research and Therapy
Volume7
Issue number5
DOIs
Publication statusPublished - 2012 Oct 8

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Nerve Regeneration
Sciatic Nerve
Peripheral Nerves
Stem Cells
Transplantation
Neural Conduction
Peripheral Nerve Injuries
Control Groups
Muscular Atrophy
Stem Cell Transplantation
Denervation
Tissue Engineering
Myelin Sheath
Contracts
Cell- and Tissue-Based Therapy
Nerve Fibers

Keywords

  • Adipose-derived stem cells(ASCs)
  • Differentiated cell
  • Nerve regeneration
  • Nerve repair
  • Neural differentiation
  • Stem cell therapy

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

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AU - Yoon, Eul Sik

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