Transplantation of rat adipose-derived stem cells (ASCs) to improve the survival of autologous aspirated fat grafts

Dae Kyun Park, Yi Hwa Ji, Eun-Sang Dhong, Ja Hea Gu, Eul Sik Yoon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although autologous lipoaspirated fat grafting is now the most commonly used technique for soft tissue augmentation, problems, such as, unpredictability and a low rate of graft survival remain. The purpose of this study was to compare the long-term viabilities of aspirated fat grafts mixed with adipose-derived stem cells (ASCs; the experimental group) and aspirated fat grafts alone (the control group). Autologous aspirated fats were taken from the interscapular area and transplanted under scalp skin with or without ASCs. Remaining fat volumes were measured at 8 and 12 weeks after transplantation. Numbers of fat cells were randomly measured by hematoxylin and eosin staining. Mean autologous fat volumes in the experimental and control groups at week 8 were 0.410±0.040 ml and 0.418±0.043 ml, respectively, and at week 12 were 0.227±0.025 ml and 0.363±0.047 ml, respectively. Adding ASCs increased the survival rate of transplanted autologous fats at week 12 (p=0.006), and many new blood vessels formed in the outer layer of transplanted tissues by gross examination. H&E staining showed that the density of adipocytes in the experimental group was significantly higher than in the control group (p=0.033). The present study suggest that ASCs improve the survival of autologous fat grafts at 12 weeks after transplantation, which supports the notion that adding ASCs at time of lipotransfer helps maintain soft tissue augmentation.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalTissue Engineering and Regenerative Medicine
Volume9
Issue number2
DOIs
Publication statusPublished - 2012 Apr 1

Fingerprint

Oils and fats
Stem cells
Grafts
Rats
Stem Cells
Transplantation
Fats
Transplants
Adipocytes
Tissue
Control Groups
Cell Survival
Staining and Labeling
Transplantation (surgical)
Graft Survival
Hematoxylin
Eosine Yellowish-(YS)
Scalp
Blood vessels
Blood Vessels

Keywords

  • Adipose-derived stem cells
  • Cell therapy
  • Fat graft
  • Lipoinjection
  • Soft tissue augmentation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Transplantation of rat adipose-derived stem cells (ASCs) to improve the survival of autologous aspirated fat grafts. / Park, Dae Kyun; Ji, Yi Hwa; Dhong, Eun-Sang; Gu, Ja Hea; Yoon, Eul Sik.

In: Tissue Engineering and Regenerative Medicine, Vol. 9, No. 2, 01.04.2012, p. 69-74.

Research output: Contribution to journalArticle

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