Triggering receptor expressed on myeloid cells 2 (TREM2) promotes adipogenesis and diet-induced obesity

Min Park, Ja Woon Yi, Eun Mi Kim, Il Joo Yoon, Eun Hee Lee, Hwa Youn Lee, Kon Young Ji, Kwang Ho Lee, Ji Hun Jang, Seung Su Oh, Chul Ho Yun, Seung Hyung Kim, Ki Mo Lee, Mun Gyu Song, Dong-Hun Kim, Hyung Sik Kang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Triggering receptor expressed onmyeloid cells 2 (TREM2) is known to be involved in the anti-inflammatory response and osteoclast development. However, the role of TREM2 in adipogenesis or obesity has not yet been defined. The effect of TREM2 on adipogenesis and obesity was investigated in TREM2 transgenic (TG) mice on a high-fat diet (HFD). To block TREM2 signaling, a neutralizing fusion protein specific for TREM2 (TREM2- Ig) was used. TG mice were much more obese than wild-type mice after feeding with an HFD, independent of the quantity of food intake. These HFD-fed TG mice manifested adipocyte hypertrophy, glucose and insulin resistance, and hepatic steatosis. The expression of adipogenic regulator genes, such as peroxisome proliferator-Activated receptor γ and CCAAT/enhancerbinding protein a, was markedly increased in HFD-fed TG mice. Additionally, HFD-fed TG mice exhibited decreased Wnt10b expression and increased GSK-3b (glycogen synthase kinase-3b)-mediated b-catenin phosphorylation. In contrast, the blockade of TREM2 signaling using TREM2-Ig resulted in the inhibition of adipocyte differentiation in vitro and a reduction in body weight in vivo by downregulating the expression of adipogenic regulators. Our data demonstrate that TREM2 promotes adipogenesis and diet-induced obesity by upregulating adipogenic regulators in conjunction with inhibiting the Wnt10b/b-catenin signaling pathway.

Original languageEnglish
Pages (from-to)117-127
Number of pages11
JournalDiabetes
Volume64
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Adipogenesis
Myeloid Cells
Obesity
Diet
High Fat Diet
Transgenic Mice
Catenins
Adipocytes
Glycogen Synthase Kinases
Obese Mice
Peroxisome Proliferator-Activated Receptors
Osteoclasts
Regulator Genes
Hypertrophy
Insulin Resistance
Proteins
Anti-Inflammatory Agents
Down-Regulation
Eating
Body Weight

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Medicine(all)

Cite this

Park, M., Yi, J. W., Kim, E. M., Yoon, I. J., Lee, E. H., Lee, H. Y., ... Kang, H. S. (2015). Triggering receptor expressed on myeloid cells 2 (TREM2) promotes adipogenesis and diet-induced obesity. Diabetes, 64(1), 117-127. https://doi.org/10.2337/db13-1869

Triggering receptor expressed on myeloid cells 2 (TREM2) promotes adipogenesis and diet-induced obesity. / Park, Min; Yi, Ja Woon; Kim, Eun Mi; Yoon, Il Joo; Lee, Eun Hee; Lee, Hwa Youn; Ji, Kon Young; Lee, Kwang Ho; Jang, Ji Hun; Oh, Seung Su; Yun, Chul Ho; Kim, Seung Hyung; Lee, Ki Mo; Song, Mun Gyu; Kim, Dong-Hun; Kang, Hyung Sik.

In: Diabetes, Vol. 64, No. 1, 01.01.2015, p. 117-127.

Research output: Contribution to journalArticle

Park, M, Yi, JW, Kim, EM, Yoon, IJ, Lee, EH, Lee, HY, Ji, KY, Lee, KH, Jang, JH, Oh, SS, Yun, CH, Kim, SH, Lee, KM, Song, MG, Kim, D-H & Kang, HS 2015, 'Triggering receptor expressed on myeloid cells 2 (TREM2) promotes adipogenesis and diet-induced obesity', Diabetes, vol. 64, no. 1, pp. 117-127. https://doi.org/10.2337/db13-1869
Park, Min ; Yi, Ja Woon ; Kim, Eun Mi ; Yoon, Il Joo ; Lee, Eun Hee ; Lee, Hwa Youn ; Ji, Kon Young ; Lee, Kwang Ho ; Jang, Ji Hun ; Oh, Seung Su ; Yun, Chul Ho ; Kim, Seung Hyung ; Lee, Ki Mo ; Song, Mun Gyu ; Kim, Dong-Hun ; Kang, Hyung Sik. / Triggering receptor expressed on myeloid cells 2 (TREM2) promotes adipogenesis and diet-induced obesity. In: Diabetes. 2015 ; Vol. 64, No. 1. pp. 117-127.
@article{4320a36db4124c5f9fc849d9af828716,
title = "Triggering receptor expressed on myeloid cells 2 (TREM2) promotes adipogenesis and diet-induced obesity",
abstract = "Triggering receptor expressed onmyeloid cells 2 (TREM2) is known to be involved in the anti-inflammatory response and osteoclast development. However, the role of TREM2 in adipogenesis or obesity has not yet been defined. The effect of TREM2 on adipogenesis and obesity was investigated in TREM2 transgenic (TG) mice on a high-fat diet (HFD). To block TREM2 signaling, a neutralizing fusion protein specific for TREM2 (TREM2- Ig) was used. TG mice were much more obese than wild-type mice after feeding with an HFD, independent of the quantity of food intake. These HFD-fed TG mice manifested adipocyte hypertrophy, glucose and insulin resistance, and hepatic steatosis. The expression of adipogenic regulator genes, such as peroxisome proliferator-Activated receptor γ and CCAAT/enhancerbinding protein a, was markedly increased in HFD-fed TG mice. Additionally, HFD-fed TG mice exhibited decreased Wnt10b expression and increased GSK-3b (glycogen synthase kinase-3b)-mediated b-catenin phosphorylation. In contrast, the blockade of TREM2 signaling using TREM2-Ig resulted in the inhibition of adipocyte differentiation in vitro and a reduction in body weight in vivo by downregulating the expression of adipogenic regulators. Our data demonstrate that TREM2 promotes adipogenesis and diet-induced obesity by upregulating adipogenic regulators in conjunction with inhibiting the Wnt10b/b-catenin signaling pathway.",
author = "Min Park and Yi, {Ja Woon} and Kim, {Eun Mi} and Yoon, {Il Joo} and Lee, {Eun Hee} and Lee, {Hwa Youn} and Ji, {Kon Young} and Lee, {Kwang Ho} and Jang, {Ji Hun} and Oh, {Seung Su} and Yun, {Chul Ho} and Kim, {Seung Hyung} and Lee, {Ki Mo} and Song, {Mun Gyu} and Dong-Hun Kim and Kang, {Hyung Sik}",
year = "2015",
month = "1",
day = "1",
doi = "10.2337/db13-1869",
language = "English",
volume = "64",
pages = "117--127",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association Inc.",
number = "1",

}

TY - JOUR

T1 - Triggering receptor expressed on myeloid cells 2 (TREM2) promotes adipogenesis and diet-induced obesity

AU - Park, Min

AU - Yi, Ja Woon

AU - Kim, Eun Mi

AU - Yoon, Il Joo

AU - Lee, Eun Hee

AU - Lee, Hwa Youn

AU - Ji, Kon Young

AU - Lee, Kwang Ho

AU - Jang, Ji Hun

AU - Oh, Seung Su

AU - Yun, Chul Ho

AU - Kim, Seung Hyung

AU - Lee, Ki Mo

AU - Song, Mun Gyu

AU - Kim, Dong-Hun

AU - Kang, Hyung Sik

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Triggering receptor expressed onmyeloid cells 2 (TREM2) is known to be involved in the anti-inflammatory response and osteoclast development. However, the role of TREM2 in adipogenesis or obesity has not yet been defined. The effect of TREM2 on adipogenesis and obesity was investigated in TREM2 transgenic (TG) mice on a high-fat diet (HFD). To block TREM2 signaling, a neutralizing fusion protein specific for TREM2 (TREM2- Ig) was used. TG mice were much more obese than wild-type mice after feeding with an HFD, independent of the quantity of food intake. These HFD-fed TG mice manifested adipocyte hypertrophy, glucose and insulin resistance, and hepatic steatosis. The expression of adipogenic regulator genes, such as peroxisome proliferator-Activated receptor γ and CCAAT/enhancerbinding protein a, was markedly increased in HFD-fed TG mice. Additionally, HFD-fed TG mice exhibited decreased Wnt10b expression and increased GSK-3b (glycogen synthase kinase-3b)-mediated b-catenin phosphorylation. In contrast, the blockade of TREM2 signaling using TREM2-Ig resulted in the inhibition of adipocyte differentiation in vitro and a reduction in body weight in vivo by downregulating the expression of adipogenic regulators. Our data demonstrate that TREM2 promotes adipogenesis and diet-induced obesity by upregulating adipogenic regulators in conjunction with inhibiting the Wnt10b/b-catenin signaling pathway.

AB - Triggering receptor expressed onmyeloid cells 2 (TREM2) is known to be involved in the anti-inflammatory response and osteoclast development. However, the role of TREM2 in adipogenesis or obesity has not yet been defined. The effect of TREM2 on adipogenesis and obesity was investigated in TREM2 transgenic (TG) mice on a high-fat diet (HFD). To block TREM2 signaling, a neutralizing fusion protein specific for TREM2 (TREM2- Ig) was used. TG mice were much more obese than wild-type mice after feeding with an HFD, independent of the quantity of food intake. These HFD-fed TG mice manifested adipocyte hypertrophy, glucose and insulin resistance, and hepatic steatosis. The expression of adipogenic regulator genes, such as peroxisome proliferator-Activated receptor γ and CCAAT/enhancerbinding protein a, was markedly increased in HFD-fed TG mice. Additionally, HFD-fed TG mice exhibited decreased Wnt10b expression and increased GSK-3b (glycogen synthase kinase-3b)-mediated b-catenin phosphorylation. In contrast, the blockade of TREM2 signaling using TREM2-Ig resulted in the inhibition of adipocyte differentiation in vitro and a reduction in body weight in vivo by downregulating the expression of adipogenic regulators. Our data demonstrate that TREM2 promotes adipogenesis and diet-induced obesity by upregulating adipogenic regulators in conjunction with inhibiting the Wnt10b/b-catenin signaling pathway.

UR - http://www.scopus.com/inward/record.url?scp=84920043974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920043974&partnerID=8YFLogxK

U2 - 10.2337/db13-1869

DO - 10.2337/db13-1869

M3 - Article

VL - 64

SP - 117

EP - 127

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 1

ER -