Trim11 increases expression of dopamine β-hydroxylase gene by interacting with Phox2b

Seok Jong Hong, Han Chae, Thomas Lardaro, Sunghoi Hong, Kwang Soo Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The homeodomain transcription factor Phox2b is one of the key determinants involved in the development of noradrenergic (NA) neurons in both the central nervous system (CNS) and the peripheral nervous system (PNS). Using yeast two-hybrid screening, we isolated a Phox2b interacting protein, Trim11, which belongs to TRIM (Tripartite motif) or RBCC proteins family, and contains a RING domain, B-boxes, a coiled-coil domain, and the B30.2/SPRY domain. Protein-protein interaction assays showed that Phox2b was able to physically interact with Trim11. The B30.2/SPRY domain of Trim11 was required for the interaction with Phox2b. Expression of Phox2b and Trim11 was detected in the sympathetic ganglia (SG) of mouse embryos. Forced expression of Trim11 with Phox2b further increased mRNA levels of dopamine β-hydroxylase (DBH) gene in primary avian neural crest stem cell (NCSC) culture. This study suggests a potential role for Trim11 in the specification of NA phenotype by interaction with Phox2b.

Original languageEnglish
Pages (from-to)650-655
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume368
Issue number3
DOIs
Publication statusPublished - 2008 Apr 11
Externally publishedYes

Keywords

  • Neural crest stem cell
  • Noradrenergic neuron
  • Phox2b
  • Protein-protein interaction
  • Trim11
  • Yeast two-hybrid screening

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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