TRP-independent inhibition of the phospholipase C pathway by natural sensory ligands

Kyung Yoon Kim, Sangsu Bang, Shanshu Han, Yen Hoang Nguyen, Tong Mook Kang, Keon Wook Kang, Sun Wook Hwang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Menthol, cinnamaldehyde, and camphor are activators for temperature-sensitive transient receptor potential ion channels (thermoTRPs). Here we found that these three compounds inhibit the phospholipase C (PLC) signaling. P2Y purinoceptor-mediated or histamine receptor-mediated cytosolic calcium mobilization through the PLC pathway was significantly suppressed by menthol, cinnamaldehyde, and camphor. Experiments using a fluorescent pleckstrin homology domain of PLCδ1 and IP1 accumulation assays demonstrated that direct inhibition of PLC activity occurred upon the addition of the sensory compounds. P2Y receptor-mediated PLC activation is part of the mechanism of platelet aggregation. The three compounds inhibited ADP-induced platelet aggregation. Calcium influx studies showed that thermoTRPs do not function in platelets, suggesting that the anti-aggregation effect is independent of thermoTRP activity. These results suggest that menthol, cinnamaldehyde, and camphor are able to modify PLC signaling and that those effects may lead to changes in cellular functions. This study also identifies new types of compounds that could potentially modulate platelet-related pathological events.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalBiochemical and biophysical research communications
Volume370
Issue number2
DOIs
Publication statusPublished - 2008 May 30

Keywords

  • Camphor
  • Cinnamaldehyde
  • Menthol
  • Phospholipase C
  • Platelet aggregation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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