TRPV1 recapitulates native capsaicin receptor in sensory neurons in association with Fas-associated factor 1

Sangsung Kim, Changjoong Kang, Young Shin Chan, Sun Wook Hwang, Duk Yang Young, Sik Shim Won, Min Young Park, Eunhee Kim, Misook Kim, Byung Moon Kim, Hawon Cho, Youngki Shin, Uhtaek Oh

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49 Citations (Scopus)

Abstract

TRPV1, a cloned capsaicin receptor, is a molecular sensor for detecting adverse stimuli and a key element for inflammatory nociception and represents biophysical properties of native channel. However, there seems to be a marked difference between TRPV1 and native capsaicin receptors in the pharmacological response profiles to vanilloids or acid. One plausible explanation for this overt discrepancy is the presence of regulatory proteins associated with TRPV1. Here, we identify Fas-associated factor 1 (FAF1) as a regulatory factor, which is coexpressed with and binds to TRPV1 in sensory neurons. When expressed heterologously, FAF1 reduces the responses of TRPV1 to capsaicin, acid, and heat, to the pharmacological level of native capsaicin receptor in sensory neurons. Furthermore, silencing FAF1 by RNA interference augments capsaicin-sensitive current in native sensory neurons. We therefore conclude that FAF1 forms an integral component of the vanilloid receptor complex and that it constitutively modulates the sensitivity of TRPV1 to various noxious stimuli in sensory neurons.

Original languageEnglish
Pages (from-to)2403-2412
Number of pages10
JournalJournal of Neuroscience
Volume26
Issue number9
DOIs
Publication statusPublished - 2006 Mar 1

Keywords

  • Association
  • Capsaicin
  • FAF1
  • Pain
  • Pharmacology
  • TRPV1

ASJC Scopus subject areas

  • Neuroscience(all)

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    Kim, S., Kang, C., Chan, Y. S., Hwang, S. W., Young, D. Y., Won, S. S., Park, M. Y., Kim, E., Kim, M., Kim, B. M., Cho, H., Shin, Y., & Oh, U. (2006). TRPV1 recapitulates native capsaicin receptor in sensory neurons in association with Fas-associated factor 1. Journal of Neuroscience, 26(9), 2403-2412. https://doi.org/10.1523/JNEUROSCI.4691-05.2006