TRPV1 recapitulates native capsaicin receptor in sensory neurons in association with Fas-associated factor 1

Sangsung Kim, Changjoong Kang, Young Shin Chan, Sun Wook Hwang, Duk Yang Young, Sik Shim Won, Min Young Park, Eunhee Kim, Misook Kim, Byung Moon Kim, Hawon Cho, Youngki Shin, Uhtaek Oh

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

TRPV1, a cloned capsaicin receptor, is a molecular sensor for detecting adverse stimuli and a key element for inflammatory nociception and represents biophysical properties of native channel. However, there seems to be a marked difference between TRPV1 and native capsaicin receptors in the pharmacological response profiles to vanilloids or acid. One plausible explanation for this overt discrepancy is the presence of regulatory proteins associated with TRPV1. Here, we identify Fas-associated factor 1 (FAF1) as a regulatory factor, which is coexpressed with and binds to TRPV1 in sensory neurons. When expressed heterologously, FAF1 reduces the responses of TRPV1 to capsaicin, acid, and heat, to the pharmacological level of native capsaicin receptor in sensory neurons. Furthermore, silencing FAF1 by RNA interference augments capsaicin-sensitive current in native sensory neurons. We therefore conclude that FAF1 forms an integral component of the vanilloid receptor complex and that it constitutively modulates the sensitivity of TRPV1 to various noxious stimuli in sensory neurons.

Original languageEnglish
Pages (from-to)2403-2412
Number of pages10
JournalJournal of Neuroscience
Volume26
Issue number9
DOIs
Publication statusPublished - 2006 Mar 1

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TRPV Cation Channels
Sensory Receptor Cells
Capsaicin
Pharmacology
Acids
Nociception
RNA Interference
Hot Temperature
Proteins

Keywords

  • Association
  • Capsaicin
  • FAF1
  • Pain
  • Pharmacology
  • TRPV1

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

TRPV1 recapitulates native capsaicin receptor in sensory neurons in association with Fas-associated factor 1. / Kim, Sangsung; Kang, Changjoong; Chan, Young Shin; Hwang, Sun Wook; Young, Duk Yang; Won, Sik Shim; Park, Min Young; Kim, Eunhee; Kim, Misook; Kim, Byung Moon; Cho, Hawon; Shin, Youngki; Oh, Uhtaek.

In: Journal of Neuroscience, Vol. 26, No. 9, 01.03.2006, p. 2403-2412.

Research output: Contribution to journalArticle

Kim, S, Kang, C, Chan, YS, Hwang, SW, Young, DY, Won, SS, Park, MY, Kim, E, Kim, M, Kim, BM, Cho, H, Shin, Y & Oh, U 2006, 'TRPV1 recapitulates native capsaicin receptor in sensory neurons in association with Fas-associated factor 1', Journal of Neuroscience, vol. 26, no. 9, pp. 2403-2412. https://doi.org/10.1523/JNEUROSCI.4691-05.2006
Kim, Sangsung ; Kang, Changjoong ; Chan, Young Shin ; Hwang, Sun Wook ; Young, Duk Yang ; Won, Sik Shim ; Park, Min Young ; Kim, Eunhee ; Kim, Misook ; Kim, Byung Moon ; Cho, Hawon ; Shin, Youngki ; Oh, Uhtaek. / TRPV1 recapitulates native capsaicin receptor in sensory neurons in association with Fas-associated factor 1. In: Journal of Neuroscience. 2006 ; Vol. 26, No. 9. pp. 2403-2412.
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