TTF-1, a homeodomain-containing transcription factor, regulates feeding behavior in the rat hypothalamus

Jae Geun Kim, Il Seong Nam-Goong, Chang Ho Yun, Jin Kwon Jeong, Eun Sook Kim, Joong Jean Park, Young Chul Lee, Young Il Kim, Byung Ju Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

TTF-1 is a member of the NKx family of homeodomain genes, and is required for morphogenesis and fetal diencephalon development. Our previous studies have shown that TTF-1 expression is maintained in some regions of the postnatal rat brain and transactivates the gene expression of several neuropeptides. In this study, a potential role for TTF-1 in the regulation of feeding behavior was identified. Immunohistochemical analysis showed that TTF-1 is present in several hypothalamic nuclei of the adult rat brain involved in the control of feeding behavior. Food deprivation for two days markedly increased the hypothalamic levels of TTF-1 mRNA and protein. Intracerebroventricular administration of an antisense TTF-1 oligodeoxynucleotide significantly decreased TTF-1 protein abundance in the hypothalamus. This TTF-1 decrease was followed by a significant decrease in neuropeptide Y mRNA content and an increase in proopiomelanocortin mRNA content, and in turn resulted in a decrease of the animal's food intake and body weight. These results suggest a novel role for TTF-1 in the regulation of feeding behavior in the rat hypothalamus.

Original languageEnglish
Pages (from-to)969-975
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume349
Issue number3
DOIs
Publication statusPublished - 2006 Oct 27

Fingerprint

Feeding Behavior
Hypothalamus
Rats
Transcription Factors
Messenger RNA
Brain
Food Deprivation
Diencephalon
Pro-Opiomelanocortin
Oligodeoxyribonucleotides
Neuropeptide Y
Fetal Development
Neuropeptides
Morphogenesis
Gene expression
Animals
Proteins
Eating
Genes
Body Weight

Keywords

  • Food intake
  • Hypothalamus
  • Neuropeptide Y
  • Obesity
  • Proopiomelanocortin
  • TTF-1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

TTF-1, a homeodomain-containing transcription factor, regulates feeding behavior in the rat hypothalamus. / Kim, Jae Geun; Nam-Goong, Il Seong; Yun, Chang Ho; Jeong, Jin Kwon; Kim, Eun Sook; Park, Joong Jean; Lee, Young Chul; Kim, Young Il; Lee, Byung Ju.

In: Biochemical and Biophysical Research Communications, Vol. 349, No. 3, 27.10.2006, p. 969-975.

Research output: Contribution to journalArticle

Kim, Jae Geun ; Nam-Goong, Il Seong ; Yun, Chang Ho ; Jeong, Jin Kwon ; Kim, Eun Sook ; Park, Joong Jean ; Lee, Young Chul ; Kim, Young Il ; Lee, Byung Ju. / TTF-1, a homeodomain-containing transcription factor, regulates feeding behavior in the rat hypothalamus. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 349, No. 3. pp. 969-975.
@article{05f4afd8118140fd854a98bd163b32f5,
title = "TTF-1, a homeodomain-containing transcription factor, regulates feeding behavior in the rat hypothalamus",
abstract = "TTF-1 is a member of the NKx family of homeodomain genes, and is required for morphogenesis and fetal diencephalon development. Our previous studies have shown that TTF-1 expression is maintained in some regions of the postnatal rat brain and transactivates the gene expression of several neuropeptides. In this study, a potential role for TTF-1 in the regulation of feeding behavior was identified. Immunohistochemical analysis showed that TTF-1 is present in several hypothalamic nuclei of the adult rat brain involved in the control of feeding behavior. Food deprivation for two days markedly increased the hypothalamic levels of TTF-1 mRNA and protein. Intracerebroventricular administration of an antisense TTF-1 oligodeoxynucleotide significantly decreased TTF-1 protein abundance in the hypothalamus. This TTF-1 decrease was followed by a significant decrease in neuropeptide Y mRNA content and an increase in proopiomelanocortin mRNA content, and in turn resulted in a decrease of the animal's food intake and body weight. These results suggest a novel role for TTF-1 in the regulation of feeding behavior in the rat hypothalamus.",
keywords = "Food intake, Hypothalamus, Neuropeptide Y, Obesity, Proopiomelanocortin, TTF-1",
author = "Kim, {Jae Geun} and Nam-Goong, {Il Seong} and Yun, {Chang Ho} and Jeong, {Jin Kwon} and Kim, {Eun Sook} and Park, {Joong Jean} and Lee, {Young Chul} and Kim, {Young Il} and Lee, {Byung Ju}",
year = "2006",
month = "10",
day = "27",
doi = "10.1016/j.bbrc.2006.08.147",
language = "English",
volume = "349",
pages = "969--975",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - TTF-1, a homeodomain-containing transcription factor, regulates feeding behavior in the rat hypothalamus

AU - Kim, Jae Geun

AU - Nam-Goong, Il Seong

AU - Yun, Chang Ho

AU - Jeong, Jin Kwon

AU - Kim, Eun Sook

AU - Park, Joong Jean

AU - Lee, Young Chul

AU - Kim, Young Il

AU - Lee, Byung Ju

PY - 2006/10/27

Y1 - 2006/10/27

N2 - TTF-1 is a member of the NKx family of homeodomain genes, and is required for morphogenesis and fetal diencephalon development. Our previous studies have shown that TTF-1 expression is maintained in some regions of the postnatal rat brain and transactivates the gene expression of several neuropeptides. In this study, a potential role for TTF-1 in the regulation of feeding behavior was identified. Immunohistochemical analysis showed that TTF-1 is present in several hypothalamic nuclei of the adult rat brain involved in the control of feeding behavior. Food deprivation for two days markedly increased the hypothalamic levels of TTF-1 mRNA and protein. Intracerebroventricular administration of an antisense TTF-1 oligodeoxynucleotide significantly decreased TTF-1 protein abundance in the hypothalamus. This TTF-1 decrease was followed by a significant decrease in neuropeptide Y mRNA content and an increase in proopiomelanocortin mRNA content, and in turn resulted in a decrease of the animal's food intake and body weight. These results suggest a novel role for TTF-1 in the regulation of feeding behavior in the rat hypothalamus.

AB - TTF-1 is a member of the NKx family of homeodomain genes, and is required for morphogenesis and fetal diencephalon development. Our previous studies have shown that TTF-1 expression is maintained in some regions of the postnatal rat brain and transactivates the gene expression of several neuropeptides. In this study, a potential role for TTF-1 in the regulation of feeding behavior was identified. Immunohistochemical analysis showed that TTF-1 is present in several hypothalamic nuclei of the adult rat brain involved in the control of feeding behavior. Food deprivation for two days markedly increased the hypothalamic levels of TTF-1 mRNA and protein. Intracerebroventricular administration of an antisense TTF-1 oligodeoxynucleotide significantly decreased TTF-1 protein abundance in the hypothalamus. This TTF-1 decrease was followed by a significant decrease in neuropeptide Y mRNA content and an increase in proopiomelanocortin mRNA content, and in turn resulted in a decrease of the animal's food intake and body weight. These results suggest a novel role for TTF-1 in the regulation of feeding behavior in the rat hypothalamus.

KW - Food intake

KW - Hypothalamus

KW - Neuropeptide Y

KW - Obesity

KW - Proopiomelanocortin

KW - TTF-1

UR - http://www.scopus.com/inward/record.url?scp=33748583493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748583493&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.08.147

DO - 10.1016/j.bbrc.2006.08.147

M3 - Article

VL - 349

SP - 969

EP - 975

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -