Tumor targeting chitosan nanoparticles for dual-modality optical/MR cancer imaging

Taehwan Nam, Sangjin Park, Seung Young Lee, Kyeongsoon Park, Kuiwon Choi, In Chan Song, Moon Hee Han, James J. Leary, Simseok Andrew Yuk, Ick Chan Kwon, Kwang Meyung Kim, Seo Young Jeong

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

We report tumor targeting nanoparticles for optical/MR dual imaging based on self-assembled glycol chitosan to be a potential multimodal imaging probe. To develop an optical/MR dual imaging probe, biocompatible and water-soluble glycol chitosan (Mw = 50 kDa) were chemically modified with 5β-cholanic acid (CA), resulting in amphiphilic glycol chitosan-5β- cholanic acid conjugates (GC-CA). For optical imaging near-infrared fluorescence (NIRF) dye, Cy5.5, was conjugated to GC-CA resulting in Cy5-labeled GC-CA conjugates (Cy5.5-GC-CA). Moreover, in order to chelate gadolinium (Gd(III)) in the Cy5.5-GC-CA conjugates, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) was directly conjugated in Cy5.5-GC-CA. Finally, the excess GdCl3 was added to DOTA modified Cy5.5-GC-CA conjugates in distilled water (pH 5.5). The freshly prepared Gd(III) encapsulated Cy5.5-GC-CA conjugates were spontaneously self-assembled into stable Cy5.5 labeled and Gd(III) encapsulated chitosan nanoparticles (Cy5.5-CNP-Gd(III)). The Cy5.5-CNP-Gd(III) was spherical in shape and approximately 350 nm in size. From the cellular experiment, it was demonstrated that Cy5.5-CNP-Gd(III) were efficiently taken up and distributed in cytoplasm (NIRF filter; red). When the Cy5.5-GC-Gd(III) were systemically administrated into the tail vein of tumor-bearing mice, large amounts of nanoparticles were successfully localized within the tumor, which was confirmed by noninvasive near-infrared fluorescence and MR imaging system simultaneously. These results revealed that the dual-modal imaging probe of Cy5.5-CNP-Gd(III) has the potential to be used as an optical/MR dual imaging agent for cancer treatment.

Original languageEnglish
Pages (from-to)578-582
Number of pages5
JournalBioconjugate Chemistry
Volume21
Issue number4
DOIs
Publication statusPublished - 2010 Apr 21
Externally publishedYes

Fingerprint

Chitosan
Nanoparticles
Glycols
Tumors
Imaging techniques
Acids
Neoplasms
Fluorescence
Optical Imaging
Infrared radiation
CY5.5 cyanine dye
Bearings (structural)
Multimodal Imaging
glycol-chitosan
cholanic acid
Oncology
Gadolinium
Water
Imaging systems
Tail

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Nam, T., Park, S., Lee, S. Y., Park, K., Choi, K., Song, I. C., ... Jeong, S. Y. (2010). Tumor targeting chitosan nanoparticles for dual-modality optical/MR cancer imaging. Bioconjugate Chemistry, 21(4), 578-582. https://doi.org/10.1021/bc900408z

Tumor targeting chitosan nanoparticles for dual-modality optical/MR cancer imaging. / Nam, Taehwan; Park, Sangjin; Lee, Seung Young; Park, Kyeongsoon; Choi, Kuiwon; Song, In Chan; Han, Moon Hee; Leary, James J.; Yuk, Simseok Andrew; Kwon, Ick Chan; Kim, Kwang Meyung; Jeong, Seo Young.

In: Bioconjugate Chemistry, Vol. 21, No. 4, 21.04.2010, p. 578-582.

Research output: Contribution to journalArticle

Nam, T, Park, S, Lee, SY, Park, K, Choi, K, Song, IC, Han, MH, Leary, JJ, Yuk, SA, Kwon, IC, Kim, KM & Jeong, SY 2010, 'Tumor targeting chitosan nanoparticles for dual-modality optical/MR cancer imaging', Bioconjugate Chemistry, vol. 21, no. 4, pp. 578-582. https://doi.org/10.1021/bc900408z
Nam, Taehwan ; Park, Sangjin ; Lee, Seung Young ; Park, Kyeongsoon ; Choi, Kuiwon ; Song, In Chan ; Han, Moon Hee ; Leary, James J. ; Yuk, Simseok Andrew ; Kwon, Ick Chan ; Kim, Kwang Meyung ; Jeong, Seo Young. / Tumor targeting chitosan nanoparticles for dual-modality optical/MR cancer imaging. In: Bioconjugate Chemistry. 2010 ; Vol. 21, No. 4. pp. 578-582.
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AU - Han, Moon Hee

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