Tumoral accumulation of long-circulating, self-assembled nanoparticles and its visualization by gamma scintigraphy

Yong Woo Cho, Yoo Shin Kim, In San Kim, Rang Woon Park, Seung Jun Oh, Dae Hyuk Moon, Sang Yoon Kim, Ick Chan Kwon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The enhanced permeability and retention (EPR) effect is used extensively for the passive targeting of many macromolecular drugs for tumors. Indeed, the EPR concept has been a gold standard in polymeric anticancer drug delivery systems. This study investigated the tumoral distribution of self-assembled nanoparticles based on the EPR effect using fluorescein and radio-labeled nanoparticles. Self-assembled nanoparticles were prepared from amphiphilic chitosan derivatives, and their tissue distribution was examined in tumor-bearing mice. The size of the nanoparticles was controlled to be 330 nm, which is a size suited for opening between thedefective endothelial cells in tumors. The long-circulating polymer nanoparticles were allowed to gradually accumulate in the tumors for 11 days. The amount of nanoparticles accumulated in the tumors was remarkably augmented from 3.4%ID/g tissue at 1 day to 25.9%ID/g tissue at 11 days after i.v. administration. The self-assembled nanoparticles were sustained at a high level throughout the 14 day experimental period, indicating their long systemic retention in the blood circulation. The γ-images provided clear evidence of selective tumor localization of the 131I-labeled nanoparticles. Confocal microscopy revealed the fluorescein-labeled nanoparticles to be preferentially localized in the perivascular regions, suggesting their extravasation to the tumors through the hyperpermeable angiogenic tumor vasculature. This highly selective tumoral accumulation of nanoparticles was attributed to the leakiness of the blood vessels in the tumors and their long residence time in the blood circulation.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalMacromolecular Research
Volume16
Issue number1
DOIs
Publication statusPublished - 2008 Jan

    Fingerprint

Keywords

  • EPR effect
  • Gamma scintigraphy
  • Long-circulating
  • Nanoparticles
  • Tumor targeting

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this