Tumours with elevated levels of the Notch and Wnt pathways exhibit efficacy to PF-03084014, a γ-secretase inhibitor, in a preclinical colorectal explant model

J. J. Arcaroli, K. S. Quackenbush, A. Purkey, R. W. Powell, T. M. Pitts, S. Bagby, A. C. Tan, B. Cross, K. McPhillips, E. K. Song, W. M. Tai, R. A. Winn, K. Bikkavilli, M. Vanscoyk, S. G. Eckhardt, W. A. Messersmith

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24 Citations (Scopus)

Abstract

Background: Dysregulation of the Notch pathway has been identified to play an important role in the development and progression of colorectal cancer (CRC). In this study, we used a patient-derived CRC explant model to investigate the efficacy of the clinical γ-secretase inhibitor (GSI) PF-03084014. Methods: A total of 16 CRC explants were treated with PF-03084014. Knockdown of RBPjκ gene was used to determine the specificity of PF-03084014. Evaluation of the Notch and Wnt pathways in CRC explant tumours was performed by gene array and immunoblotting.Results:We identified a subset of CRC tumours that exhibited elevations of the Notch and Wnt pathways sensitive to PF-03084014. Treatment with the GSI resulted in a significant reduction in cleaved Notch, Axin2 (Wnt-dependent gene) and active β-catenin. In addition, knockdown of the RBPjκ gene showed that PF-03084014 has specificity for the Notch pathway in an HCT116 cell line xenograft model. Finally, an increase in apoptosis was observed in CRC001-and CRC021-sensitive tumours. Conclusion: This study provides evidence that inhibition of γ-secretase may be beneficial in a subset of patients with elevated levels of the Wnt and Notch pathways.

Original languageEnglish
Pages (from-to)667-675
Number of pages9
JournalBritish Journal of Cancer
Volume109
Issue number3
DOIs
Publication statusPublished - 2013 Aug 6

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Arcaroli, J. J., Quackenbush, K. S., Purkey, A., Powell, R. W., Pitts, T. M., Bagby, S., Tan, A. C., Cross, B., McPhillips, K., Song, E. K., Tai, W. M., Winn, R. A., Bikkavilli, K., Vanscoyk, M., Eckhardt, S. G., & Messersmith, W. A. (2013). Tumours with elevated levels of the Notch and Wnt pathways exhibit efficacy to PF-03084014, a γ-secretase inhibitor, in a preclinical colorectal explant model. British Journal of Cancer, 109(3), 667-675. https://doi.org/10.1038/bjc.2013.361