Two chloroplast DNA inversions originated simultaneously during the early evolution of the sunflower family (Asteraceae)

Ki-Joong Kim, Keung Sun Choi, Robert K. Jansen

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

The chloroplast DNA (cpDNA) inversion in the Asteraceae has been cited as a classic example of using genomic rearrangements for defining major lineages of plants. We further characterize cpDNA inversions in the Asteraceae using extensive sequence comparisons among 56 species, including representatives of all major clades of the family and related families. We determine the boundaries of the 22-kb (now known as 22.8 kb) inversion that defines a major split within the Asteraceae, and in the process, we characterize the second and a new, smaller 3.3-kb inversion that occurs at one end of the larger inversion. One end point of the smaller inversion is upstream of the trnE-UUC gene, and the other end point is located between the trnC-GCA and rpoB genes. Although a diverse sampling of Asteraceae experienced substantial length variation and base substitution during the long evolutionary history subsequent to the inversion events, the precise locations of the inversion end points are identified using comparative sequence alignments in the inversion regions. The phylogenetic distribution of two inversions is identical among the members of Asteraceae, suggesting that the inversion events likely occurred simultaneously or within a short time period shortly after the origin of the family. Estimates of divergence times based on ndhF and rbcL sequences suggest that two inversions originated during the late Eocene (38-42 MYA). The divergence time estimates also suggest that the Asteraceae originated in the mid Eocene (42-47 MYA).

Original languageEnglish
Pages (from-to)1783-1792
Number of pages10
JournalMolecular Biology and Evolution
Volume22
Issue number9
DOIs
Publication statusPublished - 2005 Sep 1

Fingerprint

Chloroplast DNA
Asteraceae
Helianthus
chloroplast DNA
chloroplast
Helianthus annuus
Genes
DNA
Substitution reactions
Sampling
Sequence Alignment
sequence alignment
family
inversion
Eocene
genes
History
divergence
genomics
history

Keywords

  • Asteraceae
  • Chloroplast DNA inversion
  • Molecular clock
  • Nonparametric rate smoothing

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

Two chloroplast DNA inversions originated simultaneously during the early evolution of the sunflower family (Asteraceae). / Kim, Ki-Joong; Choi, Keung Sun; Jansen, Robert K.

In: Molecular Biology and Evolution, Vol. 22, No. 9, 01.09.2005, p. 1783-1792.

Research output: Contribution to journalArticle

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