Two-dimensional gel analysis of stress proteins identified in Chironomus flaviplumus (Diptera

Chironomidae) exposed to 4-nonylphenol

Myoung Chul Kim, Sung Sik Han

Research output: Contribution to journalArticle

Abstract

Toxicity of 4-nonylphenol (4-NP) in the Chironomidae Chironomus flaviplumus was analyzed using a proteomics approach that involved identifying proteins by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Proteome analysis of 4-NP-treated samples on silver stained gels found alterations in the expression levels of three proteins compared with control samples. Hsp70 proteins, so-called stress proteins, were studied in Chironomus flaviplumus exposed to different concentrations of 4-nonylphenol (0, 30, 100, 150, 300 and 600 mg/L) in the laboratory and in the field in captured animals from site 1 (1 km from a chemical factory) and site 3 (16 km from a chemical factory). Hsp70 proteins were found in all samples tested, including controls, but differed in their expression levels. At more polluted sites (site 1), the samples treated with higher concentrations of 4-NP more strongly expressed Hsp70. 2-D spots were induced or enhanced in gels following injection of 4-NP. Therefore, the induction of stress protein expression in Chironomus flaviplumus, in particular Hsp70, can be used as a biomarker for the evaluation of environmental conditions.

Original languageEnglish
Pages (from-to)164-170
Number of pages7
JournalEntomological Research
Volume40
Issue number3
DOIs
Publication statusPublished - 2010 May 1

Fingerprint

Chironomus
Chironomidae
gels
factories
proteins
polyacrylamide gel electrophoresis
sampling
two-dimensional gel electrophoresis
proteome
silver
proteomics
biomarkers
protein synthesis
toxicity
injection
environmental factors
animals

Keywords

  • 4-nonylphenol
  • Biomarker
  • Chironomus flaviplumus; two-dimensional polyacrylamide gel

ASJC Scopus subject areas

  • Insect Science

Cite this

Two-dimensional gel analysis of stress proteins identified in Chironomus flaviplumus (Diptera : Chironomidae) exposed to 4-nonylphenol. / Kim, Myoung Chul; Han, Sung Sik.

In: Entomological Research, Vol. 40, No. 3, 01.05.2010, p. 164-170.

Research output: Contribution to journalArticle

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