TY - JOUR
T1 - Two-Dimensional Microfluidic System for the Simultaneous Quantitative Analysis of Phototactic/Chemotactic Responses of Microalgae
AU - Sung, Young Joon
AU - Kwak, Ho Seok
AU - Hong, Min Eui
AU - Choi, Hong Il
AU - Sim, Sang Jun
N1 - Funding Information:
This study was supported by the Korea CCS R&D Center (Korea CCS 2020 Project) of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT of Korea (Grant No. 2014M1A8A1049278), the NRF (Grant No. NRF-2016R1A2A1A05005465) the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korean government (Ministry of Trade, Industry and Energy) (Grant No. 20172010202050), and the Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (Grant No. KDHC-1003542018S075), with special thanks to Korea University (Seoul, Korea).
Funding Information:
This study was supported by the Korea CCS R&D Center (Korea CCS 2020 Project) of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT of Korea (Grant No. 2014M1A8A1049278), the NRF (Grant No. NRF-2016R1A2A1A05005465), the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korean government (Ministry of Trade, Industry and Energy) (Grant No. 20172010202050), and the Future Development Institute grant funded by Korea District Heating Corporation (KDHC) (Grant No. KDHC-1003542018S075), with special thanks to Korea University (Seoul, Korea).
Publisher Copyright:
© Copyright 2018 American Chemical Society.
PY - 2018/12/4
Y1 - 2018/12/4
N2 - Microalgae have been spotlighted as a renewable energy source to produce biofuels from CO 2 by photosynthesis. However, their innate inefficiency of CO 2 conversion using light energy has been a challenge to the commercialization of algae-based biofuel production. Photosynthetic organisms have evolved behavioral responses, including phototaxis and chemotaxis, to find optimal conditions for capturing light energy and inorganic carbon (Ci) sources for photosynthesis. In this context, investigation of phototaxis and chemotaxis to HCO 3 - , the predominant form of Ci in neutral aqueous solutions, is necessary to understand the physiological role of tactic responses in photosynthesis. In this study, a two-dimensional microfluidic system enabled efficient analysis of phototactic and chemotactic responses by investigation of cell distribution in the outlet chambers. From statistical analysis (skewness and kurtosis) of tactic responses of different algal strains to external stimuli, the preferred concentrations of HCO 3 - for Chlamydomonas reinhardtii CC125 (wild type), PTS42 (random insertional mutant of C. reinhardtii, high photosynthetic activity), and CC2702 (cia5 mutant of C. reinhardtii, unable to acclimate to low CO 2 concentration) were determined to be 27.22, 43.23, and 36.95 mM, respectively. From the analysis of tactic responses of wild type and 14 mutant strains, it was found that the photosystem II (PSII) operating efficiency and CO 2 fixation rate were strongly correlated with the phototactic (R 2 = 0.931) and chemotactic response (R 2 = 0.857), respectively. Finally, this system can be applied to high-throughput screening strategies for the rapid isolation of high photosynthetically productive microalgal strains based on their tactic responses.
AB - Microalgae have been spotlighted as a renewable energy source to produce biofuels from CO 2 by photosynthesis. However, their innate inefficiency of CO 2 conversion using light energy has been a challenge to the commercialization of algae-based biofuel production. Photosynthetic organisms have evolved behavioral responses, including phototaxis and chemotaxis, to find optimal conditions for capturing light energy and inorganic carbon (Ci) sources for photosynthesis. In this context, investigation of phototaxis and chemotaxis to HCO 3 - , the predominant form of Ci in neutral aqueous solutions, is necessary to understand the physiological role of tactic responses in photosynthesis. In this study, a two-dimensional microfluidic system enabled efficient analysis of phototactic and chemotactic responses by investigation of cell distribution in the outlet chambers. From statistical analysis (skewness and kurtosis) of tactic responses of different algal strains to external stimuli, the preferred concentrations of HCO 3 - for Chlamydomonas reinhardtii CC125 (wild type), PTS42 (random insertional mutant of C. reinhardtii, high photosynthetic activity), and CC2702 (cia5 mutant of C. reinhardtii, unable to acclimate to low CO 2 concentration) were determined to be 27.22, 43.23, and 36.95 mM, respectively. From the analysis of tactic responses of wild type and 14 mutant strains, it was found that the photosystem II (PSII) operating efficiency and CO 2 fixation rate were strongly correlated with the phototactic (R 2 = 0.931) and chemotactic response (R 2 = 0.857), respectively. Finally, this system can be applied to high-throughput screening strategies for the rapid isolation of high photosynthetically productive microalgal strains based on their tactic responses.
UR - http://www.scopus.com/inward/record.url?scp=85057521380&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.8b04121
DO - 10.1021/acs.analchem.8b04121
M3 - Article
C2 - 30411621
AN - SCOPUS:85057521380
VL - 90
SP - 14029
EP - 14038
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 23
ER -