Two dinucleotide repeat polymorphisms (AC/TG and GT/CA) in the 5' upstream region of the mouse tryptophan hydroxylase gene

S. V. Yim, S. G. Chi, S. H. Chung, H. J. Lee, M. J. Kim, S. J. Park, C. Jung, J. H. Chung

Research output: Contribution to journalArticle

Abstract

Tryptophan hydroxylase (TPH), the rate-limiting enzyme in serotonin biosynthesis, is primarily expressed in serotonergic neurons of the raphe nuclei. Simple tandem repeat polymorphisms, typically one to four nucleotides long, are tandemly repeated several times and often characterized by many alleles. To identify the presence of polymorphic repeats, we sequenced the 5'-upstream region of the mouse TPH gene. For the detection of any allelic variants, polymerase chain reaction, nonisotopic single-strand conformation polymorphism, and DNA sequencing analyses of the tandem repeat sequences were performed using genomic DNA extracted from 60 ICR mice. Two dinucleotide repeats, 5'-(AC/TG)22-3' and 5'-(GT/CA)17-3', were identified at approximately -5.7 kb and -3.4 kb upstream from the transcriptional initiation site of the mouse TPH gene, respectively. Minor allelic variants, 5'-(AC/TG)21-3' and 5'-(GT/CA)18-3', were observed in heterozygous pairs from 3 of 60 and 1 of 60 ICR mice, respectively. The identification of these microsatellites in the mouse TPH promoter raises the possibility that identical and/or other polymorphic sequences might exist in the upstream region of the human TPH gene.

Original languageEnglish
Pages (from-to)501-505
Number of pages5
JournalKorean Journal of Physiology and Pharmacology
Volume3
Issue number5
Publication statusPublished - 1999

Keywords

  • AC repeats
  • Dinucleotide polymorphism
  • GT repeats
  • Microsatellite
  • Mouse tryptophan hydroxylase (TPH)

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

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