Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue

Myung Kim Hwan; Ha Jeong Byeong; Ju Yong Hyon; Jin An Myoung; Sik Seo Mun; Hee Hong Jin; Kyoung J. Lee; Hoon Kim Chul; Taiha Joo; Seok Cheol Hong; Rae Cho Bong

doi:10.1021/ja711391f

Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue

Myung Kim Hwan, Ha Jeong Byeong, Ju Yong Hyon, Jin An Myoung, Sik Seo Mun, Hee Hong Jin, Kyoung J. Lee, Hoon Kim Chul, Taiha Joo, Seok Cheol Hong, Rae Cho Bong

Research output: Contribution to journal › Article › peer-review

120 Citations (Scopus)

Abstract

We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 μm in live tissues using two-photon microscopy.

Original language	English
Pages (from-to)	4246-4247
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	130
Issue number	13
DOIs	https://doi.org/10.1021/ja711391f
Publication status	Published - 2008 Apr 2

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja711391f

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abstract = "We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 μm in live tissues using two-photon microscopy.",

author = "Hwan, {Myung Kim} and Byeong, {Ha Jeong} and Hyon, {Ju Yong} and Myoung, {Jin An} and Mun, {Sik Seo} and Jin, {Hee Hong} and Lee, {Kyoung J.} and Chul, {Hoon Kim} and Taiha Joo and Hong, {Seok Cheol} and Bong, {Rae Cho}",

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AU - Hwan, Myung Kim

AU - Byeong, Ha Jeong

AU - Hyon, Ju Yong

AU - Myoung, Jin An

AU - Mun, Sik Seo

AU - Jin, Hee Hong

AU - Lee, Kyoung J.

AU - Chul, Hoon Kim

AU - Joo, Taiha

AU - Hong, Seok Cheol

AU - Bong, Rae Cho

PY - 2008/4/2

Y1 - 2008/4/2

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AB - We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 μm in live tissues using two-photon microscopy.

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Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue

Abstract

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