Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue

Myung Kim Hwan, Ha Jeong Byeong, Ju Yong Hyon, Jin An Myoung, Sik Seo Mun, Hee Hong Jin, Kyoung Jin Lee, Hoon Kim Chul, Taiha Joo, Seok Cheol Hong, Bong-Rae Cho

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 μm in live tissues using two-photon microscopy.

Original languageEnglish
Pages (from-to)4246-4247
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number13
DOIs
Publication statusPublished - 2008 Apr 2

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Photons
Lipids
Tissue
Fluorescence
Pyramidal Cells
Naphthalene
Neurons
Microscopy
Microscopic examination
Visualization
Cells

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Hwan, M. K., Byeong, H. J., Hyon, J. Y., Myoung, J. A., Mun, S. S., Jin, H. H., ... Cho, B-R. (2008). Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue. Journal of the American Chemical Society, 130(13), 4246-4247. https://doi.org/10.1021/ja711391f

Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue. / Hwan, Myung Kim; Byeong, Ha Jeong; Hyon, Ju Yong; Myoung, Jin An; Mun, Sik Seo; Jin, Hee Hong; Lee, Kyoung Jin; Chul, Hoon Kim; Joo, Taiha; Hong, Seok Cheol; Cho, Bong-Rae.

In: Journal of the American Chemical Society, Vol. 130, No. 13, 02.04.2008, p. 4246-4247.

Research output: Contribution to journalArticle

Hwan, MK, Byeong, HJ, Hyon, JY, Myoung, JA, Mun, SS, Jin, HH, Lee, KJ, Chul, HK, Joo, T, Hong, SC & Cho, B-R 2008, 'Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue', Journal of the American Chemical Society, vol. 130, no. 13, pp. 4246-4247. https://doi.org/10.1021/ja711391f
Hwan, Myung Kim ; Byeong, Ha Jeong ; Hyon, Ju Yong ; Myoung, Jin An ; Mun, Sik Seo ; Jin, Hee Hong ; Lee, Kyoung Jin ; Chul, Hoon Kim ; Joo, Taiha ; Hong, Seok Cheol ; Cho, Bong-Rae. / Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue. In: Journal of the American Chemical Society. 2008 ; Vol. 130, No. 13. pp. 4246-4247.
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