Two-photon probe for Cu2+ with an internal reference: Quantitative estimation of Cu2+ in human tissues by two-photon microscopy

Dong Eun Kang; Chang Su Lim; Ji Yeon Kim; Eun Sun Kim; Hoon Jai Chun; Bong Rae Cho

doi:10.1021/ac500329k

Two-photon probe for Cu²⁺ with an internal reference: Quantitative estimation of Cu²⁺ in human tissues by two-photon microscopy

Dong Eun Kang, Chang Su Lim, Ji Yeon Kim, Eun Sun Kim, Hoon Jai Chun, Bong Rae Cho

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

Copper ions play a crucial role in living systems as cofactors of numerous metalloenzymes. To quantitatively estimate the Cu²⁺ concentration in human tissue, we have developed a two-photon (TP) probe with an internal reference (ACCu2) that shows significant TP action cross-section and high selectivity for Cu²⁺ and can quantitatively estimate the Cu ²⁺ concentration in human colon tissues by dual-color two-photon microscopy (TPM) imaging with minimum interference from other competing metal ions or pH and minimum cytotoxicity and photostability problems. The Cu ²⁺ concentrations in human normal colon, polyp, and colon cancer tissues were found to be 8.3 ± 0.3, 13 ± 2, and 22 ± 3 μM, respectively. This result suggests that ACCu2 may be useful for the diagnosis of human colon cancer.

Original language	English
Pages (from-to)	5353-5359
Number of pages	7
Journal	Analytical chemistry
Volume	86
Issue number	11
DOIs	https://doi.org/10.1021/ac500329k
Publication status	Published - 2014 Jun 3
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/ac500329k

Cite this

@article{70adac69777e45da974a272ddd697b33,

title = "Two-photon probe for Cu2+ with an internal reference: Quantitative estimation of Cu2+ in human tissues by two-photon microscopy",

abstract = "Copper ions play a crucial role in living systems as cofactors of numerous metalloenzymes. To quantitatively estimate the Cu2+ concentration in human tissue, we have developed a two-photon (TP) probe with an internal reference (ACCu2) that shows significant TP action cross-section and high selectivity for Cu2+ and can quantitatively estimate the Cu 2+ concentration in human colon tissues by dual-color two-photon microscopy (TPM) imaging with minimum interference from other competing metal ions or pH and minimum cytotoxicity and photostability problems. The Cu 2+ concentrations in human normal colon, polyp, and colon cancer tissues were found to be 8.3 ± 0.3, 13 ± 2, and 22 ± 3 μM, respectively. This result suggests that ACCu2 may be useful for the diagnosis of human colon cancer.",

author = "Kang, {Dong Eun} and Lim, {Chang Su} and Kim, {Ji Yeon} and Kim, {Eun Sun} and Chun, {Hoon Jai} and Cho, {Bong Rae}",

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T1 - Two-photon probe for Cu2+ with an internal reference

T2 - Quantitative estimation of Cu2+ in human tissues by two-photon microscopy

AU - Kang, Dong Eun

AU - Lim, Chang Su

AU - Kim, Ji Yeon

AU - Kim, Eun Sun

AU - Chun, Hoon Jai

AU - Cho, Bong Rae

PY - 2014/6/3

Y1 - 2014/6/3

N2 - Copper ions play a crucial role in living systems as cofactors of numerous metalloenzymes. To quantitatively estimate the Cu2+ concentration in human tissue, we have developed a two-photon (TP) probe with an internal reference (ACCu2) that shows significant TP action cross-section and high selectivity for Cu2+ and can quantitatively estimate the Cu 2+ concentration in human colon tissues by dual-color two-photon microscopy (TPM) imaging with minimum interference from other competing metal ions or pH and minimum cytotoxicity and photostability problems. The Cu 2+ concentrations in human normal colon, polyp, and colon cancer tissues were found to be 8.3 ± 0.3, 13 ± 2, and 22 ± 3 μM, respectively. This result suggests that ACCu2 may be useful for the diagnosis of human colon cancer.

AB - Copper ions play a crucial role in living systems as cofactors of numerous metalloenzymes. To quantitatively estimate the Cu2+ concentration in human tissue, we have developed a two-photon (TP) probe with an internal reference (ACCu2) that shows significant TP action cross-section and high selectivity for Cu2+ and can quantitatively estimate the Cu 2+ concentration in human colon tissues by dual-color two-photon microscopy (TPM) imaging with minimum interference from other competing metal ions or pH and minimum cytotoxicity and photostability problems. The Cu 2+ concentrations in human normal colon, polyp, and colon cancer tissues were found to be 8.3 ± 0.3, 13 ± 2, and 22 ± 3 μM, respectively. This result suggests that ACCu2 may be useful for the diagnosis of human colon cancer.

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