Two-photon probe for Cu2+ with an internal reference: Quantitative estimation of Cu2+ in human tissues by two-photon microscopy

Dong Eun Kang, Chang Su Lim, Ji Yeon Kim, Eun Sun Kim, Hoon Jai Chun, Bong Rae Cho

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62 Citations (Scopus)


Copper ions play a crucial role in living systems as cofactors of numerous metalloenzymes. To quantitatively estimate the Cu2+ concentration in human tissue, we have developed a two-photon (TP) probe with an internal reference (ACCu2) that shows significant TP action cross-section and high selectivity for Cu2+ and can quantitatively estimate the Cu 2+ concentration in human colon tissues by dual-color two-photon microscopy (TPM) imaging with minimum interference from other competing metal ions or pH and minimum cytotoxicity and photostability problems. The Cu 2+ concentrations in human normal colon, polyp, and colon cancer tissues were found to be 8.3 ± 0.3, 13 ± 2, and 22 ± 3 μM, respectively. This result suggests that ACCu2 may be useful for the diagnosis of human colon cancer.

Original languageEnglish
Pages (from-to)5353-5359
Number of pages7
JournalAnalytical chemistry
Issue number11
Publication statusPublished - 2014 Jun 3

ASJC Scopus subject areas

  • Analytical Chemistry


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