Two-Photon Probes for Golgi Apparatus

Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy

Ji Woo Choi, Seung Taek Hong, Mun Seok Kim, Kyu Cheol Paik, Man So Han, Bong-Rae Cho

Research output: Contribution to journalArticle

Abstract

We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10 -50 cm 4 ·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity.

Original languageEnglish
JournalAnalytical Chemistry
DOIs
Publication statusPublished - 2019 Jan 1

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Microscopic examination
Photons
Tissue
Pyrazines
Fluorophores
Cytotoxicity
Fluorescence
Derivatives
Wavelength
Peptides

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Two-Photon Probes for Golgi Apparatus : Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy. / Choi, Ji Woo; Hong, Seung Taek; Kim, Mun Seok; Paik, Kyu Cheol; Han, Man So; Cho, Bong-Rae.

In: Analytical Chemistry, 01.01.2019.

Research output: Contribution to journalArticle

Choi, Ji Woo ; Hong, Seung Taek ; Kim, Mun Seok ; Paik, Kyu Cheol ; Han, Man So ; Cho, Bong-Rae. / Two-Photon Probes for Golgi Apparatus : Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy. In: Analytical Chemistry. 2019.
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