Two-Photon Probes for Lysosomes and Mitochondria: Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging

Chang Su Lim, Seung Taek Hong, Seong Shick Ryu, Dong Eun Kang, Bong-Rae Cho

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Novel two-photon (TP) probes were developed for lysosomes (PLT-yellow) and mitochondria (BMT-blue and PMT-yellow). These probes emitted strong TP-excited fluorescence in cells at widely separated wavelength regions and displayed high organelle selectivity, good cell permeability, low cytotoxicity, and pH insensitivity. The BMT-blue and PLT-yellow probes could be utilized to detect lysosomes and mitochondria simultaneously in live tissues by using dual-color two-photon microscopy, with minimum interference from each other.

Original languageEnglish
Pages (from-to)2240-2249
Number of pages10
JournalChemistry - An Asian Journal
Volume10
Issue number10
DOIs
Publication statusPublished - 2015 Oct 1

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Mitochondria
Microscopic examination
Photons
Tissue
Color
Imaging techniques
Cytotoxicity
Fluorescence
Wavelength

Keywords

  • fluorescent probes
  • imaging agents
  • lysosomes
  • mitochondria
  • two photon

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Two-Photon Probes for Lysosomes and Mitochondria : Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging. / Lim, Chang Su; Hong, Seung Taek; Ryu, Seong Shick; Kang, Dong Eun; Cho, Bong-Rae.

In: Chemistry - An Asian Journal, Vol. 10, No. 10, 01.10.2015, p. 2240-2249.

Research output: Contribution to journalArticle

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