Two-Photon Probes for pH: Detection of Human Colon Cancer using Two-Photon Microscopy

Seung Taek Hong, Tae Hyeong Kim, Ji Woo Choi, Seong Jun Park, Sung An Kwon, Kyu Cheol Paik, Man So Han, Eun-Sun Kim, Hoon-Jai Chun, Jung Nyoung Heo, Bong-Rae Cho

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8 Citations (Scopus)


We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10-50cm4s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (Pacid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger Pacid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating much brighter TPM images and 6-fold larger Pacid values compared to normal tissue. These results suggest the potential utility of BHEt-1 for detecting colon cancer in human tissues using TPM.

Original languageEnglish
Pages (from-to)9830-9835
Number of pages6
JournalAnalytical Chemistry
Issue number18
Publication statusPublished - 2017 Sep 19

ASJC Scopus subject areas

  • Analytical Chemistry

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