Abstract
We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10-50cm4s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (Pacid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger Pacid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating much brighter TPM images and 6-fold larger Pacid values compared to normal tissue. These results suggest the potential utility of BHEt-1 for detecting colon cancer in human tissues using TPM.
| Original language | English |
|---|---|
| Pages (from-to) | 9830-9835 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 89 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - 2017 Sep 19 |
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Cite this
Two-Photon Probes for pH : Detection of Human Colon Cancer using Two-Photon Microscopy. / Hong, Seung Taek; Kim, Tae Hyeong; Choi, Ji Woo; Park, Seong Jun; Kwon, Sung An; Paik, Kyu Cheol; Han, Man So; Kim, Eun-Sun; Chun, Hoon-Jai; Heo, Jung Nyoung; Cho, Bong-Rae.
In: Analytical Chemistry, Vol. 89, No. 18, 19.09.2017, p. 9830-9835.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two-Photon Probes for pH
T2 - Detection of Human Colon Cancer using Two-Photon Microscopy
AU - Hong, Seung Taek
AU - Kim, Tae Hyeong
AU - Choi, Ji Woo
AU - Park, Seong Jun
AU - Kwon, Sung An
AU - Paik, Kyu Cheol
AU - Han, Man So
AU - Kim, Eun-Sun
AU - Chun, Hoon-Jai
AU - Heo, Jung Nyoung
AU - Cho, Bong-Rae
PY - 2017/9/19
Y1 - 2017/9/19
N2 - We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10-50cm4s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (Pacid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger Pacid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating much brighter TPM images and 6-fold larger Pacid values compared to normal tissue. These results suggest the potential utility of BHEt-1 for detecting colon cancer in human tissues using TPM.
AB - We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10-50cm4s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (Pacid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger Pacid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating much brighter TPM images and 6-fold larger Pacid values compared to normal tissue. These results suggest the potential utility of BHEt-1 for detecting colon cancer in human tissues using TPM.
UR - http://www.scopus.com/inward/record.url?scp=85029573629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029573629&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.7b01804
DO - 10.1021/acs.analchem.7b01804
M3 - Article
C2 - 28806509
AN - SCOPUS:85029573629
VL - 89
SP - 9830
EP - 9835
JO - Industrial And Engineering Chemistry Analytical Edition
JF - Industrial And Engineering Chemistry Analytical Edition
SN - 0003-2700
IS - 18
ER -