Two-Photon Probes for pH

Detection of Human Colon Cancer using Two-Photon Microscopy

Seung Taek Hong, Tae Hyeong Kim, Ji Woo Choi, Seong Jun Park, Sung An Kwon, Kyu Cheol Paik, Man So Han, Eun-Sun Kim, Hoon-Jai Chun, Jung Nyoung Heo, Bong-Rae Cho

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed two-photon (TP) pH-sensitive probes (BH-2 and BHEt-1) that exhibit absorption and emission maxima at 370 and 466 nm, and TP absorption cross-section values of 51 and 61 GM (1 GM = 10-50cm4s/photon), respectively, at 750 nm and pH 3.0 in a universal buffer (0.1 M citric acid, 0.1 M KH2PO4, 0.1 M Na2B4O7, 0.1 M Tris, 0.1 M KCl)/1,4-dioxane (7/3) solution. The TPM images of CCD-18co (a normal colon cell line) and HCT116 cells (a colon cancer cell line) labeled with BH-2 were too dim to be distinguished. When the same cells were labeled with BHEt-1, however, the TPM image of the HCT116 cells was much brighter than that of CCD-18co cells, and the relative proportion of the acidic vesicles (Pacid) of the former was 5-fold larger than that of latter. BHEt-1 could also differentiate HepG2 cells (a human liver cancer cell line) from LX-2 cells (a human hepatic stellate cell line) with a 6-fold larger Pacid value. Human colon cancer tissues labeled with BHEt-1 showed similar results, demonstrating much brighter TPM images and 6-fold larger Pacid values compared to normal tissue. These results suggest the potential utility of BHEt-1 for detecting colon cancer in human tissues using TPM.

Original languageEnglish
Pages (from-to)9830-9835
Number of pages6
JournalAnalytical Chemistry
Volume89
Issue number18
DOIs
Publication statusPublished - 2017 Sep 19

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Microscopic examination
Photons
Cells
Tissue
Charge coupled devices
Citric Acid
Liver
Buffers
1,4-dioxane
Hepatic Stellate Cells

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Two-Photon Probes for pH : Detection of Human Colon Cancer using Two-Photon Microscopy. / Hong, Seung Taek; Kim, Tae Hyeong; Choi, Ji Woo; Park, Seong Jun; Kwon, Sung An; Paik, Kyu Cheol; Han, Man So; Kim, Eun-Sun; Chun, Hoon-Jai; Heo, Jung Nyoung; Cho, Bong-Rae.

In: Analytical Chemistry, Vol. 89, No. 18, 19.09.2017, p. 9830-9835.

Research output: Contribution to journalArticle

Hong, Seung Taek ; Kim, Tae Hyeong ; Choi, Ji Woo ; Park, Seong Jun ; Kwon, Sung An ; Paik, Kyu Cheol ; Han, Man So ; Kim, Eun-Sun ; Chun, Hoon-Jai ; Heo, Jung Nyoung ; Cho, Bong-Rae. / Two-Photon Probes for pH : Detection of Human Colon Cancer using Two-Photon Microscopy. In: Analytical Chemistry. 2017 ; Vol. 89, No. 18. pp. 9830-9835.
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AU - Kwon, Sung An

AU - Paik, Kyu Cheol

AU - Han, Man So

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