Two-photon probes for Zn2+ ions with various dissociation constants. Detection of Zn2+ ions in live cells and tissues by two-photon microscopy

Isravel Antony Danish, Chang Su Lim, Yu Shun Tian, Ji Hee Han, Min Young Kang, Bong Rae Cho

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

A series of Zn2+-selective two-photon fluorescent probes (AZnM1-AZnN) that had a wide range of dissociation constants (Kd TP=8 nm-12 νM) were synthesized. These probes showed appreciable water solubility (>3 νM), cell permeability, high photostability, pH insensitivity at pH>7, significant two-photon action cross-sections (86-110 GM) upon complexation with Zn2+, and can detect the Zn2+ ions in HeLa cells and in living tissue slices of rat hippocampal at a depth of >80 νm without mistargeting and photobleaching problems. These probes can potentially find application in the detection of various amounts of Zn 2+ ions in live cells and intact tissues. A-tissue a-tissue, we all fall down: A series of Zn2+-ion selective two-photon fluorescent probes with a wide range of dissociation constants (KdTP=8 nM-12 νM) were developed to be able to detect the Zn2+ ions in live cells and intact tissues at a depth of>80 νm without the drawbacks of mistargeting and photobleaching.

Original languageEnglish
Pages (from-to)1234-1240
Number of pages7
JournalChemistry - An Asian Journal
Volume6
Issue number5
DOIs
Publication statusPublished - 2011 May 2

Keywords

  • fluorescence
  • fluorescent probes
  • live tissue
  • two-photon microscopy
  • zinc

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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