Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue

Ji Woo Choi, Seung Taek Hong, Dong Eun Kang, Kyu Cheol Paik, Man So Han, Chang Su Lim, Bong-Rae Cho

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.

Original languageEnglish
Pages (from-to)9412-9418
Number of pages7
JournalAnalytical Chemistry
Volume88
Issue number19
DOIs
Publication statusPublished - 2016 Oct 4

ASJC Scopus subject areas

  • Analytical Chemistry

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