Abstract
We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.
| Original language | English |
|---|---|
| Pages (from-to) | 9412-9418 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 88 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - 2016 Oct 4 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2 : Detection of Breast Cancer in a Live Tissue. / Choi, Ji Woo; Hong, Seung Taek; Kang, Dong Eun; Paik, Kyu Cheol; Han, Man So; Lim, Chang Su; Cho, Bong-Rae.
In: Analytical Chemistry, Vol. 88, No. 19, 04.10.2016, p. 9412-9418.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2
T2 - Detection of Breast Cancer in a Live Tissue
AU - Choi, Ji Woo
AU - Hong, Seung Taek
AU - Kang, Dong Eun
AU - Paik, Kyu Cheol
AU - Han, Man So
AU - Lim, Chang Su
AU - Cho, Bong-Rae
PY - 2016/10/4
Y1 - 2016/10/4
N2 - We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.
AB - We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.
UR - http://www.scopus.com/inward/record.url?scp=84989872106&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84989872106&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.6b00912
DO - 10.1021/acs.analchem.6b00912
M3 - Article
VL - 88
SP - 9412
EP - 9418
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 19
ER -