Abstract
We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.
| Original language | English |
|---|---|
| Pages (from-to) | 9412-9418 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 88 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - 2016 Oct 4 |
ASJC Scopus subject areas
- Analytical Chemistry
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Choi, J. W., Hong, S. T., Kang, D. E., Paik, K. C., Han, M. S., Lim, C. S., & Cho, B-R. (2016). Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue. Analytical Chemistry, 88(19), 9412-9418. https://doi.org/10.1021/acs.analchem.6b00912