TY - JOUR
T1 - Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2
T2 - Detection of Breast Cancer in a Live Tissue
AU - Choi, Ji Woo
AU - Hong, Seung Taek
AU - Kang, Dong Eun
AU - Paik, Kyu Cheol
AU - Han, Man So
AU - Lim, Chang Su
AU - Cho, Bong-Rae
PY - 2016/10/4
Y1 - 2016/10/4
N2 - We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.
AB - We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.
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U2 - 10.1021/acs.analchem.6b00912
DO - 10.1021/acs.analchem.6b00912
M3 - Article
C2 - 27599354
AN - SCOPUS:84989872106
VL - 88
SP - 9412
EP - 9418
JO - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
JF - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
SN - 0730-6512
IS - 19
ER -
