Abstract
The mechanism by which activated ras oncogene expression leads to repression of genes encoding specific actin filament proteins is not understood. However, these changes associated with loss of organized actin filaments, are necessary to maintain the transformed phenotype. The human smooth muscle (sm) α-actin promoter is repressed in ras-transformed fibroblast cells and derepressed in revertant cell lines. In this study, we demonstrate that two serum response elements (SREs) present in the α-actin promoter are required for transcriptional repression in ras-transformed cells and the two SREs act synergistically to repress heterologous promoters in a ras-transformation dependent manner. Serum response factor (SRF), which can bind to the sm α-actin SREs, restores α-actin promoter activity in ras-transformed cells. c-Fos, c-Jun and YY1 also repress α-actin promoter through SREs, suggesting that these transcription factors may play a role in repressing α-actin promoter in ras-transformed cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1361-1370 |
| Number of pages | 10 |
| Journal | Oncogene |
| Volume | 10 |
| Issue number | 7 |
| Publication status | Published - 1995 Jan 1 |
| Externally published | Yes |
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Keywords
- α-actin promoter
- ras-oncogene
- Repression
- SRES
ASJC Scopus subject areas
- Cancer Research
- Genetics
- Molecular Biology
Cite this
Two serum response elements mediate transcriptional repression of human smooth muscle α-actin promoter in ras-transformed cells. / Bushel, P.; Kim, Jae-Hong; Chang, W.; Catino, J. J.; Ruley, H. E.; Kumar, C. C.
In: Oncogene, Vol. 10, No. 7, 01.01.1995, p. 1361-1370.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two serum response elements mediate transcriptional repression of human smooth muscle α-actin promoter in ras-transformed cells
AU - Bushel, P.
AU - Kim, Jae-Hong
AU - Chang, W.
AU - Catino, J. J.
AU - Ruley, H. E.
AU - Kumar, C. C.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - The mechanism by which activated ras oncogene expression leads to repression of genes encoding specific actin filament proteins is not understood. However, these changes associated with loss of organized actin filaments, are necessary to maintain the transformed phenotype. The human smooth muscle (sm) α-actin promoter is repressed in ras-transformed fibroblast cells and derepressed in revertant cell lines. In this study, we demonstrate that two serum response elements (SREs) present in the α-actin promoter are required for transcriptional repression in ras-transformed cells and the two SREs act synergistically to repress heterologous promoters in a ras-transformation dependent manner. Serum response factor (SRF), which can bind to the sm α-actin SREs, restores α-actin promoter activity in ras-transformed cells. c-Fos, c-Jun and YY1 also repress α-actin promoter through SREs, suggesting that these transcription factors may play a role in repressing α-actin promoter in ras-transformed cells.
AB - The mechanism by which activated ras oncogene expression leads to repression of genes encoding specific actin filament proteins is not understood. However, these changes associated with loss of organized actin filaments, are necessary to maintain the transformed phenotype. The human smooth muscle (sm) α-actin promoter is repressed in ras-transformed fibroblast cells and derepressed in revertant cell lines. In this study, we demonstrate that two serum response elements (SREs) present in the α-actin promoter are required for transcriptional repression in ras-transformed cells and the two SREs act synergistically to repress heterologous promoters in a ras-transformation dependent manner. Serum response factor (SRF), which can bind to the sm α-actin SREs, restores α-actin promoter activity in ras-transformed cells. c-Fos, c-Jun and YY1 also repress α-actin promoter through SREs, suggesting that these transcription factors may play a role in repressing α-actin promoter in ras-transformed cells.
KW - α-actin promoter
KW - ras-oncogene
KW - Repression
KW - SRES
UR - http://www.scopus.com/inward/record.url?scp=0028942867&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028942867&partnerID=8YFLogxK
M3 - Article
C2 - 7731687
AN - SCOPUS:0028942867
VL - 10
SP - 1361
EP - 1370
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 7
ER -