Two serum response elements mediate transcriptional repression of human smooth muscle α-actin promoter in ras-transformed cells

P. Bushel, Jae-Hong Kim, W. Chang, J. J. Catino, H. E. Ruley, C. C. Kumar

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The mechanism by which activated ras oncogene expression leads to repression of genes encoding specific actin filament proteins is not understood. However, these changes associated with loss of organized actin filaments, are necessary to maintain the transformed phenotype. The human smooth muscle (sm) α-actin promoter is repressed in ras-transformed fibroblast cells and derepressed in revertant cell lines. In this study, we demonstrate that two serum response elements (SREs) present in the α-actin promoter are required for transcriptional repression in ras-transformed cells and the two SREs act synergistically to repress heterologous promoters in a ras-transformation dependent manner. Serum response factor (SRF), which can bind to the sm α-actin SREs, restores α-actin promoter activity in ras-transformed cells. c-Fos, c-Jun and YY1 also repress α-actin promoter through SREs, suggesting that these transcription factors may play a role in repressing α-actin promoter in ras-transformed cells.

Original languageEnglish
Pages (from-to)1361-1370
Number of pages10
JournalOncogene
Volume10
Issue number7
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Fingerprint

Serum Response Element
Smooth Muscle
Actins
Actin Cytoskeleton
Serum Response Factor
ras Genes
Transcription Factors
Fibroblasts
Phenotype
Cell Line
Genes

Keywords

  • α-actin promoter
  • ras-oncogene
  • Repression
  • SRES

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Two serum response elements mediate transcriptional repression of human smooth muscle α-actin promoter in ras-transformed cells. / Bushel, P.; Kim, Jae-Hong; Chang, W.; Catino, J. J.; Ruley, H. E.; Kumar, C. C.

In: Oncogene, Vol. 10, No. 7, 01.01.1995, p. 1361-1370.

Research output: Contribution to journalArticle

Bushel, P. ; Kim, Jae-Hong ; Chang, W. ; Catino, J. J. ; Ruley, H. E. ; Kumar, C. C. / Two serum response elements mediate transcriptional repression of human smooth muscle α-actin promoter in ras-transformed cells. In: Oncogene. 1995 ; Vol. 10, No. 7. pp. 1361-1370.
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