Two-stage cultivation of two Chlorella sp. strains by simultaneous treatment of brewery wastewater and maximizing lipid productivity

Wasif Farooq, Young Chul Lee, Byung Gon Ryu, Byung Hyuk Kim, Hee Sik Kim, Yoon-E Choi, Ji Won Yang

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102 Citations (Scopus)


A cultivation system in the two-stage photoautotrophic-photoheterotrophic/mixotrophic mode was adapted to maximize lipid productivity of two freshwater strains of Chlorella sp. grown in brewery wastewater (BWW). The endogenous Chlorella sp. isolated from BWW had a higher growth rate than wild-type Chlorella vulgaris (UTEX-265) while C. vulgaris (UTEX-265) had a higher maximal biomass and lipid contents than that of endogenous Chlorella sp., resulting in more than 90% of the inorganic nutrients in both total nitrogen (TN) and phosphorus (TP) was removed during the first stage in the two-stage photoautotrophic-photoheterotrophic mode in each Chlorella sp. The maximal biomass and lipid contents of C. vulgaris (UTEX-265) for single stage photoautotrophic cultivation were 1.5. g/L and 18%, respectively. Importantly, during two-stage photoautotrophic-photoheterotrophic cultivation for C. vulgaris (UTEX-265), the biomass was increased to 3.5. g/L, and the lipid productivity was increased from 31.1 to 108.0. mg/L. day.

Original languageEnglish
Pages (from-to)230-238
Number of pages9
JournalBioresource Technology
Publication statusPublished - 2013 Jan 1
Externally publishedYes



  • Contamination control
  • Lipid productivity
  • Microalgal biodiesel
  • Two-stage cultivation
  • Wastewater treatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

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