Two sweetpotato ADP-glucose pyrophosphorylase isoforms are regulated antagonistically in response to sucrose content in storage roots

Man Sup Kwak, Seol Ah Noh, Mi Joung Oh, Gyung Hye Huh, Kyung Nam Kim, Shin Woo Lee, Jeong Sheop Shin, Jung Myung Bae

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The transcriptional regulation of ADP-glucose pyrophosphorylase (AGPase) genes in detached leaves in response to exogenous sucrose has been investigated earlier; however the effects of endogenous sucrose on AGPase gene transcription in leaves or starch-accumulating tissues have not yet been determined. We therefore have investigated the relationship between endogenous sucrose content in the storage tissues of sweetpotato (Ipomoea batatas cv. Yulmi) and the rate of transcription of the two sweetpotato AGPase isoforms, ibAGP1 and ibAGP2, by means of transient expression analysis of their promoters. Sequence analysis of the two promoters identified putative sucrose-responsive elements on the ibAGP1 promoter and, conversely, putative sucrose-starvation elements on the ibAGP2 promoter. Transient expression analyses on transverse storage root sections revealed that the ibAGP1 and ibAGP2 promoters directed strong expression in the sweetpotato storage roots (diameter: 1.5 cm). Sucrose contents of the sweetpotato storage roots were positively correlated with growth of the storage root. In the storage roots, ibAGP1 promoter activity became stronger with increasing endogenous sucrose levels, while ibAGP2 promoter activity became markedly weaker. Consequently, ibAGP2 was expressed primarily during the early stages of storage root development, whereas ibAGP1 was abundantly expressed in the later stages, during which a profound level of starch accumulation occurs. The antagonistic regulation of the two promoters in response to endogenous sucrose levels was also confirmed in carrot (Daucus carota L. cv. Hapa-ochon) taproots.

Original languageEnglish
Pages (from-to)87-96
Number of pages10
JournalGene
Volume366
Issue number1
DOIs
Publication statusPublished - 2006 Jan 17

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Glucose-1-Phosphate Adenylyltransferase
Sucrose
Protein Isoforms
Daucus carota
Starch
Ipomoea batatas
Starvation
Genes
Sequence Analysis

Keywords

  • β-glucuronidase (GUS)
  • AGPase
  • Ipomoea batatas
  • Transcriptional regulation
  • Transient expression

ASJC Scopus subject areas

  • Genetics

Cite this

Two sweetpotato ADP-glucose pyrophosphorylase isoforms are regulated antagonistically in response to sucrose content in storage roots. / Kwak, Man Sup; Noh, Seol Ah; Oh, Mi Joung; Huh, Gyung Hye; Kim, Kyung Nam; Lee, Shin Woo; Shin, Jeong Sheop; Bae, Jung Myung.

In: Gene, Vol. 366, No. 1, 17.01.2006, p. 87-96.

Research output: Contribution to journalArticle

Kwak, Man Sup ; Noh, Seol Ah ; Oh, Mi Joung ; Huh, Gyung Hye ; Kim, Kyung Nam ; Lee, Shin Woo ; Shin, Jeong Sheop ; Bae, Jung Myung. / Two sweetpotato ADP-glucose pyrophosphorylase isoforms are regulated antagonistically in response to sucrose content in storage roots. In: Gene. 2006 ; Vol. 366, No. 1. pp. 87-96.
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abstract = "The transcriptional regulation of ADP-glucose pyrophosphorylase (AGPase) genes in detached leaves in response to exogenous sucrose has been investigated earlier; however the effects of endogenous sucrose on AGPase gene transcription in leaves or starch-accumulating tissues have not yet been determined. We therefore have investigated the relationship between endogenous sucrose content in the storage tissues of sweetpotato (Ipomoea batatas cv. Yulmi) and the rate of transcription of the two sweetpotato AGPase isoforms, ibAGP1 and ibAGP2, by means of transient expression analysis of their promoters. Sequence analysis of the two promoters identified putative sucrose-responsive elements on the ibAGP1 promoter and, conversely, putative sucrose-starvation elements on the ibAGP2 promoter. Transient expression analyses on transverse storage root sections revealed that the ibAGP1 and ibAGP2 promoters directed strong expression in the sweetpotato storage roots (diameter: 1.5 cm). Sucrose contents of the sweetpotato storage roots were positively correlated with growth of the storage root. In the storage roots, ibAGP1 promoter activity became stronger with increasing endogenous sucrose levels, while ibAGP2 promoter activity became markedly weaker. Consequently, ibAGP2 was expressed primarily during the early stages of storage root development, whereas ibAGP1 was abundantly expressed in the later stages, during which a profound level of starch accumulation occurs. The antagonistic regulation of the two promoters in response to endogenous sucrose levels was also confirmed in carrot (Daucus carota L. cv. Hapa-ochon) taproots.",
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AU - Kim, Kyung Nam

AU - Lee, Shin Woo

AU - Shin, Jeong Sheop

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