Type VI secretion is a major virulence determinant in Burkholderia mallei

Mark A. Schell, Ricky L. Ulrich, Wilson J. Ribot, Ernst E. Brueggemann, Harry B. Hines, Dan Chen, Lyla Lipscomb, Heenam Kim, Jan Mrázek, William C. Nierman, David DeShazer

Research output: Contribution to journalArticle

234 Citations (Scopus)

Abstract

Burkholderia mallei is a host-adapted pathogen and a category B biothreat agent. Although the B. mallei VirAG two-component regulatory system is required for virulence in hamsters, the virulence genes it regulates are unknown. Here we show with expression profiling that overexpression of virAG resulted in transcriptional activation of ∼60 genes, including some involved in capsule production, actin-based intracellular motility, and type VI secretion (T6S). The 15 genes encoding the major sugar component of the homopolymeric capsule were up-expressed >2.5-fold, but capsule was still produced in the absence of virAG. Actin tail formation required virAG as well as bimB, bimC and bimE, three previously uncharacterized genes that were activated four- to 15-fold when VirAG was overproduced. Surprisingly, actin polymerization was found to be dispensable for virulence in hamsters. In contrast, genes encoding a T6S system were up-expressed as much as 30-fold and mutations in this T6S gene cluster resulted in strains that were avirulent in hamsters. SDS-PAGE and mass spectrometry demonstrated that BMAA0742 was secreted by the T6S system when virAG was overexpressed. Purified His-tagged BMAA0742 was recognized by glanders antiserum from a horse, a human and mice, indicating that this Hcp-family protein is produced in vivo during infection.

Original languageEnglish
Pages (from-to)1466-1485
Number of pages20
JournalMolecular Microbiology
Volume64
Issue number6
DOIs
Publication statusPublished - 2007 Jun 1

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Burkholderia mallei
Virulence
Cricetinae
Capsules
Actins
Genes
Glanders
Multigene Family
Polymerization
Transcriptional Activation
Horses
Tail
Immune Sera
Polyacrylamide Gel Electrophoresis
Mass Spectrometry
Mutation
Infection

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Schell, M. A., Ulrich, R. L., Ribot, W. J., Brueggemann, E. E., Hines, H. B., Chen, D., ... DeShazer, D. (2007). Type VI secretion is a major virulence determinant in Burkholderia mallei. Molecular Microbiology, 64(6), 1466-1485. https://doi.org/10.1111/j.1365-2958.2007.05734.x

Type VI secretion is a major virulence determinant in Burkholderia mallei. / Schell, Mark A.; Ulrich, Ricky L.; Ribot, Wilson J.; Brueggemann, Ernst E.; Hines, Harry B.; Chen, Dan; Lipscomb, Lyla; Kim, Heenam; Mrázek, Jan; Nierman, William C.; DeShazer, David.

In: Molecular Microbiology, Vol. 64, No. 6, 01.06.2007, p. 1466-1485.

Research output: Contribution to journalArticle

Schell, MA, Ulrich, RL, Ribot, WJ, Brueggemann, EE, Hines, HB, Chen, D, Lipscomb, L, Kim, H, Mrázek, J, Nierman, WC & DeShazer, D 2007, 'Type VI secretion is a major virulence determinant in Burkholderia mallei', Molecular Microbiology, vol. 64, no. 6, pp. 1466-1485. https://doi.org/10.1111/j.1365-2958.2007.05734.x
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Molecular Microbiology. 2007 Jun 1;64(6):1466-1485. https://doi.org/10.1111/j.1365-2958.2007.05734.x
Schell, Mark A. ; Ulrich, Ricky L. ; Ribot, Wilson J. ; Brueggemann, Ernst E. ; Hines, Harry B. ; Chen, Dan ; Lipscomb, Lyla ; Kim, Heenam ; Mrázek, Jan ; Nierman, William C. ; DeShazer, David. / Type VI secretion is a major virulence determinant in Burkholderia mallei. In: Molecular Microbiology. 2007 ; Vol. 64, No. 6. pp. 1466-1485.
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