Tyrokinase inhibitor from the flowers of impatiens balsamina

Young Hee Lim, In Hwan Kim, Jung Ju Seo, Jeong Keun Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Kaempferol was isolated and identified from the methanol extract of the flowers of Impatiens balsamina. Kaempferol showed inhibitory activity against mushroom tyrosinase with an ID50 of 0.042 mM. Inhibition kinetics, as determined using a Lineweaver-Burk plot, showed kaempferol to be a competitive inhibitor of mushroom tyrosinase with a Ki value of 0.011 mM. The lag phase of tyrosine hydroxylation catalyzed by mushroom tyrosinase clearly increased on increasing the concentration of kaempferol. In addition to its tyrosinase inhibiting activity, kaempferol strongly inhibited melanin production by Streptomyces bikiniensis, in a dose-dependent manner, without inhibiting cell growth. For comparative purposes, the tyrosinase inhibitory activity of kaempferol was also assayed versus quercetin, a positive standard.

Original languageEnglish
Pages (from-to)1977-1983
Number of pages7
JournalJournal of microbiology and biotechnology
Volume16
Issue number12
Publication statusPublished - 2006 Dec

Keywords

  • Impatiens balsamina
  • Kaempferol
  • Melanin
  • Tyrosinase inhibitor

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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