Tyrosine phosphorylation of phospholipase C-γ1 by vaccinia virus growth factor

Hee Sook Kim, Young Han Lee, Do Sik Min, Jong Soo Chang, Sung Ho Ryu, Byung-Yoon Ahn, Pann Ghill Suh

Research output: Contribution to journalArticle

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Abstract

Vaccinia virus growth factor (VGF), a highly glycosylated polypeptide encoded in the genome of vaccinia virus, shares amino acid sequence homology and functional properties with cellular growth factors, ECF and TGF-α. Although the mitogenic activity of the purified or synthetic VGF suggested that the factor may be beneficial for viral replication by stimulation of host cell growth, neither the role in virus life cycle nor the step next to the EGF receptor activation had been firmly established. We found tyrosine-phosphorylations of PLC-γ1 and a concomitant increase of the phosphoinositides level in the human epidermoidal A431 cells either treated with purified VGF or infected with vaccinia virus. In contrast to the wild-type virus, a VGF- mutant virus did not induce tyrosine phosphorylation of PLC-γ1. Phosphopeptide analysis indicated that the phosphorylation of the PLC-γ1 by VGF includes tyrosine residues identical to those phosphorylated by EGF. These results suggest that tyrosine phosphorylation of PLC-γ1, mediated by VGF, leads to activation of PLC-γ1 and a concomitant increase in phosphatidylinositol turnover.

Original languageEnglish
Pages (from-to)21-28
Number of pages8
JournalVirology
Volume214
Issue number1
DOIs
Publication statusPublished - 1995 Dec 19

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Type C Phospholipases
Tyrosine
Phosphorylation
Viruses
Intercellular Signaling Peptides and Proteins
Vaccinia virus
Phosphatidylinositols
Amino Acid Sequence Homology
Phosphopeptides
vaccinia growth factor
Life Cycle Stages
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Genome
Peptides
Growth

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Kim, H. S., Lee, Y. H., Min, D. S., Chang, J. S., Ryu, S. H., Ahn, B-Y., & Suh, P. G. (1995). Tyrosine phosphorylation of phospholipase C-γ1 by vaccinia virus growth factor. Virology, 214(1), 21-28. https://doi.org/10.1006/viro.1995.9958

Tyrosine phosphorylation of phospholipase C-γ1 by vaccinia virus growth factor. / Kim, Hee Sook; Lee, Young Han; Min, Do Sik; Chang, Jong Soo; Ryu, Sung Ho; Ahn, Byung-Yoon; Suh, Pann Ghill.

In: Virology, Vol. 214, No. 1, 19.12.1995, p. 21-28.

Research output: Contribution to journalArticle

Kim, HS, Lee, YH, Min, DS, Chang, JS, Ryu, SH, Ahn, B-Y & Suh, PG 1995, 'Tyrosine phosphorylation of phospholipase C-γ1 by vaccinia virus growth factor', Virology, vol. 214, no. 1, pp. 21-28. https://doi.org/10.1006/viro.1995.9958
Kim, Hee Sook ; Lee, Young Han ; Min, Do Sik ; Chang, Jong Soo ; Ryu, Sung Ho ; Ahn, Byung-Yoon ; Suh, Pann Ghill. / Tyrosine phosphorylation of phospholipase C-γ1 by vaccinia virus growth factor. In: Virology. 1995 ; Vol. 214, No. 1. pp. 21-28.
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