Tyrosine Phosphorylation of Phospholipase C-γ1 by Vaccinia Virus Growth Factor

Hee Sook Kim, Young Han Lee, Do Sik Min, Jong Soo Chang, Sung Ho Ryu, Byung Yoon Ahn, Pann Ghill Suh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Vaccinia virus growth factor (VGF), a highly glycosylated polypeptide encoded in the genome of vaccinia virus, shares amino acid sequence homology and functional properties with cellular growth factors, EGF and TGF-α. Although the mitogenic activity of the purified or synthetic VGF suggested that the factor may be beneficial for viral replication by stimulation of host cell growth, neither the role in virus life cycle nor the step next to the EGF receptor activation had been firmly established. We found tyrosine-phosphorylations of PLC-γ1 and a concomitant increase of the phosphoinositides level in the human epidermoidal A431 cells either treated with purified VGF or infected with vaccinia virus. In contrast to the wild-type virus, a VGF-mutant virus did not induce tyrosine phosphorylation of PLC-γ1. Phosphopeptide analysis indicated that the phosphorylation of the PLC-γ1 by VGF includes tyrosine residues identical to those phosphorylated by EGF. These results suggest that tyrosine phosphorylation of PLC-γ1, mediated by VGF, leads to activation of PLC-γ1 and a concomitant increase in phosphatidylinositol turnover.

Original languageEnglish
Article number79958
Pages (from-to)21-28
Number of pages8
JournalVirology
Volume214
Issue number1
DOIs
Publication statusPublished - 1995 Dec 1

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Tyrosine Phosphorylation of Phospholipase C-γ1 by Vaccinia Virus Growth Factor'. Together they form a unique fingerprint.

  • Cite this

    Kim, H. S., Lee, Y. H., Min, D. S., Chang, J. S., Ryu, S. H., Ahn, B. Y., & Suh, P. G. (1995). Tyrosine Phosphorylation of Phospholipase C-γ1 by Vaccinia Virus Growth Factor. Virology, 214(1), 21-28. [79958]. https://doi.org/10.1006/viro.1995.9958