Ubiquinone synthesis by Pneumocystis carinii

Incorporation of radiolabeled polyprenyl chain and benzoquinone ring precursors

Dong Geun Sul, E. S. Kaneshiro

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Radiolabeled mevalonic acid was incorporated in vitro into Pneumocystis carinii ubiquinone homologs, CoQ9 and CoQ10, demonstrating the isoprenoid branch pathway forming the polyprenyl chain of ubiquinone is functional in the organism. Radiolabeled shikimic acid, tyrosine and p-hydroxybenzoic acid were also incorporated into the two ubiquinone homologs, indicating that P. carinii also possessed the biochemical pathway for de novo synthesis of the CoQ benzoquinone ring.

Original languageEnglish
JournalJournal of Eukaryotic Microbiology
Volume44
Issue number6
Publication statusPublished - 1997 Nov 1
Externally publishedYes

Fingerprint

Pneumocystis carinii
benzoquinones
Ubiquinone
ubiquinones
coenzyme Q10
synthesis
Shikimic Acid
mevalonic acid
shikimic acid
Hydroxybenzoates
Mevalonic Acid
4-hydroxybenzoic acid
isoprenoids
Terpenes
Tyrosine
tyrosine
biochemical pathways
organisms
benzoquinone

Keywords

  • Atovavaquone
  • Chorisnate
  • Mevalonate
  • p-hydroxybenzoate
  • Shikimate
  • Tyrosine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

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T2 - Incorporation of radiolabeled polyprenyl chain and benzoquinone ring precursors

AU - Sul, Dong Geun

AU - Kaneshiro, E. S.

PY - 1997/11/1

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AB - Radiolabeled mevalonic acid was incorporated in vitro into Pneumocystis carinii ubiquinone homologs, CoQ9 and CoQ10, demonstrating the isoprenoid branch pathway forming the polyprenyl chain of ubiquinone is functional in the organism. Radiolabeled shikimic acid, tyrosine and p-hydroxybenzoic acid were also incorporated into the two ubiquinone homologs, indicating that P. carinii also possessed the biochemical pathway for de novo synthesis of the CoQ benzoquinone ring.

KW - Atovavaquone

KW - Chorisnate

KW - Mevalonate

KW - p-hydroxybenzoate

KW - Shikimate

KW - Tyrosine

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