Ultra-Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for the Determination of Ketoconazole in Anti-Dandruff Shampoo

So Hyun Kim, Anil Shrestha, Nguyen Huu Hoang, Nguyen Lan Huong, Je Won Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A fast and highly sensitive method for the quantification of ketoconazole in antidandruff emulsion formulas was developed and validated. Sample preparation utilizing solid-phase extraction was a simple and reliable method for extracting both ketoconazole and its internal standard miconazole from the samples: 97 ± 3% for ketoconazole and 93 ± 4% for the internal standard. The separation by isocratic ultra-high performance liquid chromatography and multiple-stage mass spectrometry with a reversed-phase C18 column was performed within 10 min. The method, which operated in a selective reaction monitoring mode specific to ketoconazole, was validated for quantitative use. The intra- and inter-day precision values were <5% and their accuracies ranged from 89.6 to 96.2%. The ketoconazole concentrations in two samples obtained by the established protocol were comparable to the concentrations indicated on the labels of the formulations. Thus, this paper describes the first use of ultra-high performance liquid chromatography and multiple-stage mass spectrometry for the determination of ketoconazole.

Original languageEnglish
Pages (from-to)1465-1475
Number of pages11
JournalAnalytical Letters
Volume47
Issue number9
DOIs
Publication statusPublished - 2014 Jun

Keywords

  • Anti-dandruff emulsion formulations
  • Ketoconazole
  • Solid-phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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