Ultra-specific enrichment of gst-tagged protein by gsh-modified nanoparticles

Yeon Ji Lee, Jong Moon Park, Jiyoung Huh, Minsik Kim, Je Sun Lee, Arudra Palani, Kwangyeol Lee, Sang Won Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione- modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/ MS/MS experiments.

Original languageEnglish
Pages (from-to)1568-1572
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume31
Issue number6
DOIs
Publication statusPublished - 2010 Jun 20

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Keywords

  • Glutathione
  • Nanoparticle
  • Proteomics
  • Separation
  • Superparamagnetic

ASJC Scopus subject areas

  • Chemistry(all)

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