Ultra-structural analysis of the brain in a Drosophila model of Alzheimer's disease using FIB/SEM microscopy.

Se Jin Park, Andreas Schertel, Kyung Eun Lee, Sung Sik Han

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Alzheimer's disease (AD), one of the most prevalent neurodegenerative brain diseases, has been extensively researched for years. However, its synaptic structure, which is a basis for understanding neurodegenerative disorders, has not yet been understood clearly. Defining the structures of neurons and their synaptic connections is the significant goal of brain research. To study synaptic connectivity, three-dimensional (3D) reconstructions of the nervous system are very helpful. In this study, the 3D structure of brain synapses in the Drosophila melanogaster Swedish amyloid precursor protein (APP) mutant, which is characterized by early onset AD, was analyzed using focused ion beam/scanning electron microscopy (FIB/SEM). This technique is one of the most useful for 3D reconstruction, as the process of obtaining serial images is fully automated and thus avoids the problems inherent in hand-operated ultrathin serial sectioning. The 3D images of normal and AD brains reported in this study reveal characteristic features of AD such as appearance of autophagy, abnormal axon formation and increased mitochondrial size. This 3D analysis reveals structural change as a basis for understanding neurodegenerative disorder.

Original languageEnglish
Pages (from-to)3-13
Number of pages11
JournalMicroscopy (Oxford, England)
Volume63
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1

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Drosophila
Focused ion beams
structural analysis
Structural analysis
Electron Scanning Microscopy
brain
Microscopy
Brain
Microscopic examination
Alzheimer Disease
ion beams
Neurodegenerative Diseases
Ions
microscopy
Scanning electron microscopy
scanning electron microscopy
Mitochondrial Size
Amyloid beta-Protein Precursor
disorders
Autophagy

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ultra-structural analysis of the brain in a Drosophila model of Alzheimer's disease using FIB/SEM microscopy. / Park, Se Jin; Schertel, Andreas; Lee, Kyung Eun; Han, Sung Sik.

In: Microscopy (Oxford, England), Vol. 63, No. 1, 01.01.2014, p. 3-13.

Research output: Contribution to journalArticle

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