Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex

Inhong Kim, Kwangseuk Kyhm, Mijeong Kang, Han Young Woo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalJournal of Luminescence
Volume149
DOIs
Publication statusPublished - 2014 May 1
Externally publishedYes

Fingerprint

Single-Stranded DNA
Energy Transfer
Fluorescein
Energy transfer
Quenching
deoxyribonucleic acid
Fluorescence
energy transfer
quenching
Radiation
fluorescence
Fluorophores
dynamic models
encounters
Dynamic models
Boundary conditions
boundary conditions
Kinetics
kinetics
radiation

Keywords

  • Conjugated polymer
  • Exciton
  • Fluorescence quenching model
  • Förster resonance energy transfer (FRET)
  • Time-resolved fluorescence

ASJC Scopus subject areas

  • Biophysics
  • Atomic and Molecular Physics, and Optics
  • Chemistry(all)
  • Biochemistry
  • Condensed Matter Physics

Cite this

Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. / Kim, Inhong; Kyhm, Kwangseuk; Kang, Mijeong; Woo, Han Young.

In: Journal of Luminescence, Vol. 149, 01.05.2014, p. 185-189.

Research output: Contribution to journalArticle

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abstract = "The combined dynamic process of F{\"o}rster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of F{\"o}rster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 {\AA}) in comparison with the F{\"o}rster distance (43 {\AA}).",
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AB - The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).

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