Abstract
The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).
| Original language | English |
|---|---|
| Pages (from-to) | 185-189 |
| Number of pages | 5 |
| Journal | Journal of Luminescence |
| Volume | 149 |
| DOIs | |
| Publication status | Published - 2014 May 1 |
| Externally published | Yes |
Fingerprint
Keywords
- Conjugated polymer
- Exciton
- Fluorescence quenching model
- Förster resonance energy transfer (FRET)
- Time-resolved fluorescence
ASJC Scopus subject areas
- Biophysics
- Atomic and Molecular Physics, and Optics
- Chemistry(all)
- Biochemistry
- Condensed Matter Physics
Cite this
Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. / Kim, Inhong; Kyhm, Kwangseuk; Kang, Mijeong; Woo, Han Young.
In: Journal of Luminescence, Vol. 149, 01.05.2014, p. 185-189.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex
AU - Kim, Inhong
AU - Kyhm, Kwangseuk
AU - Kang, Mijeong
AU - Woo, Han Young
PY - 2014/5/1
Y1 - 2014/5/1
N2 - The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).
AB - The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).
KW - Conjugated polymer
KW - Exciton
KW - Fluorescence quenching model
KW - Förster resonance energy transfer (FRET)
KW - Time-resolved fluorescence
UR - http://www.scopus.com/inward/record.url?scp=84893504426&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893504426&partnerID=8YFLogxK
U2 - 10.1016/j.jlumin.2014.01.042
DO - 10.1016/j.jlumin.2014.01.042
M3 - Article
AN - SCOPUS:84893504426
VL - 149
SP - 185
EP - 189
JO - Journal of Luminescence
JF - Journal of Luminescence
SN - 0022-2313
ER -