Ultrasensitive detection of fibrinogen using erythrocyte membrane-draped electrochemical impedance biosensor

Joohyung Park; Wonseok Lee; Insu Kim; Minwoo Kim; Seongjae Jo; Woong Kim; Hyunjun Park; Gyudo Lee; Wonjoon Choi; Dae Sung Yoon; Jinsung Park

doi:10.1016/j.snb.2019.05.016

Ultrasensitive detection of fibrinogen using erythrocyte membrane-draped electrochemical impedance biosensor

Joohyung Park, Wonseok Lee, Insu Kim, Minwoo Kim, Seongjae Jo, Woong Kim, Hyunjun Park, Gyudo Lee, Wonjoon Choi, Dae Sung Yoon, Jinsung Park

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

Because fibrinogen plays an important role as a biomarker of many diseases, including blood coagulation-related and cardiovascular diseases, various methods for the detection of fibrinogen have been developed. Herein, we developed a simple and ultrasensitive electrochemical impedance biosensor (EIB)by draping an erythrocyte membrane (EM)for the detection of fibrinogen. Measurements with the EIB revealed that the specific (selective)adsorption of fibrinogen onto the EM caused a clear rise in the value of interfacial charge transfer resistance. The sensing ability of the EIB for fibrinogen detection showed a wide linear range from 0.0001 to 5 mg/mL, with a limit of detection of 49 ng/mL (144 pM). Moreover, the EIB was able to analyze the fibrinogen in the serum sample, which suggests that it is a facile, low-cost, and ultrasensitive method with great potential for use in the quantitative detection of fibrinogen.

Original language	English
Pages (from-to)	296-303
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	293
DOIs	https://doi.org/10.1016/j.snb.2019.05.016
Publication status	Published - 2019 Aug 15

Keywords

Charge transfer resistance
Electrochemical biosensor
Electrochemical impedance spectroscopy
Erythrocyte membrane
Fibrinogen

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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Park, J., Lee, W., Kim, I., Kim, M., Jo, S., Kim, W., Park, H., Lee, G., Choi, W., Yoon, D. S., & Park, J. (2019). Ultrasensitive detection of fibrinogen using erythrocyte membrane-draped electrochemical impedance biosensor. Sensors and Actuators, B: Chemical, 293, 296-303. https://doi.org/10.1016/j.snb.2019.05.016

Ultrasensitive detection of fibrinogen using erythrocyte membrane-draped electrochemical impedance biosensor. / Park, Joohyung; Lee, Wonseok; Kim, Insu; Kim, Minwoo; Jo, Seongjae; Kim, Woong; Park, Hyunjun; Lee, Gyudo; Choi, Wonjoon ; Yoon, Dae Sung; Park, Jinsung.

In: Sensors and Actuators, B: Chemical, Vol. 293, 15.08.2019, p. 296-303.

Research output: Contribution to journal › Article

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abstract = "Because fibrinogen plays an important role as a biomarker of many diseases, including blood coagulation-related and cardiovascular diseases, various methods for the detection of fibrinogen have been developed. Herein, we developed a simple and ultrasensitive electrochemical impedance biosensor (EIB)by draping an erythrocyte membrane (EM)for the detection of fibrinogen. Measurements with the EIB revealed that the specific (selective)adsorption of fibrinogen onto the EM caused a clear rise in the value of interfacial charge transfer resistance. The sensing ability of the EIB for fibrinogen detection showed a wide linear range from 0.0001 to 5 mg/mL, with a limit of detection of 49 ng/mL (144 pM). Moreover, the EIB was able to analyze the fibrinogen in the serum sample, which suggests that it is a facile, low-cost, and ultrasensitive method with great potential for use in the quantitative detection of fibrinogen.",

keywords = "Charge transfer resistance, Electrochemical biosensor, Electrochemical impedance spectroscopy, Erythrocyte membrane, Fibrinogen",

author = "Joohyung Park and Wonseok Lee and Insu Kim and Minwoo Kim and Seongjae Jo and Woong Kim and Hyunjun Park and Gyudo Lee and Wonjoon Choi and Yoon, {Dae Sung} and Jinsung Park",

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AB - Because fibrinogen plays an important role as a biomarker of many diseases, including blood coagulation-related and cardiovascular diseases, various methods for the detection of fibrinogen have been developed. Herein, we developed a simple and ultrasensitive electrochemical impedance biosensor (EIB)by draping an erythrocyte membrane (EM)for the detection of fibrinogen. Measurements with the EIB revealed that the specific (selective)adsorption of fibrinogen onto the EM caused a clear rise in the value of interfacial charge transfer resistance. The sensing ability of the EIB for fibrinogen detection showed a wide linear range from 0.0001 to 5 mg/mL, with a limit of detection of 49 ng/mL (144 pM). Moreover, the EIB was able to analyze the fibrinogen in the serum sample, which suggests that it is a facile, low-cost, and ultrasensitive method with great potential for use in the quantitative detection of fibrinogen.

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