TY - JOUR
T1 - Ultrasensitive detection of fibrinogen using erythrocyte membrane-draped electrochemical impedance biosensor
AU - Park, Joohyung
AU - Lee, Wonseok
AU - Kim, Insu
AU - Kim, Minwoo
AU - Jo, Seongjae
AU - Kim, Woong
AU - Park, Hyunjun
AU - Lee, Gyudo
AU - Choi, Wonjoon
AU - Yoon, Dae Sung
AU - Park, Jinsung
N1 - Funding Information:
This research was financially supported by the National Research Foundation of Korea (NRF) under Grant Nos. NRF- 2017R1E1A1A01075439 , NRF- 2017M3A9F1031229 , NRF- 2019R1C1C1005668 , and the Ministry of SMEs and Startups (MSS), Korea, under the “Regional Specialized Industry Development Program (R&D, P0005002 )” supervised by the Korea Institute for Advancement of Technology (KIAT).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/8/15
Y1 - 2019/8/15
N2 - Because fibrinogen plays an important role as a biomarker of many diseases, including blood coagulation-related and cardiovascular diseases, various methods for the detection of fibrinogen have been developed. Herein, we developed a simple and ultrasensitive electrochemical impedance biosensor (EIB)by draping an erythrocyte membrane (EM)for the detection of fibrinogen. Measurements with the EIB revealed that the specific (selective)adsorption of fibrinogen onto the EM caused a clear rise in the value of interfacial charge transfer resistance. The sensing ability of the EIB for fibrinogen detection showed a wide linear range from 0.0001 to 5 mg/mL, with a limit of detection of 49 ng/mL (144 pM). Moreover, the EIB was able to analyze the fibrinogen in the serum sample, which suggests that it is a facile, low-cost, and ultrasensitive method with great potential for use in the quantitative detection of fibrinogen.
AB - Because fibrinogen plays an important role as a biomarker of many diseases, including blood coagulation-related and cardiovascular diseases, various methods for the detection of fibrinogen have been developed. Herein, we developed a simple and ultrasensitive electrochemical impedance biosensor (EIB)by draping an erythrocyte membrane (EM)for the detection of fibrinogen. Measurements with the EIB revealed that the specific (selective)adsorption of fibrinogen onto the EM caused a clear rise in the value of interfacial charge transfer resistance. The sensing ability of the EIB for fibrinogen detection showed a wide linear range from 0.0001 to 5 mg/mL, with a limit of detection of 49 ng/mL (144 pM). Moreover, the EIB was able to analyze the fibrinogen in the serum sample, which suggests that it is a facile, low-cost, and ultrasensitive method with great potential for use in the quantitative detection of fibrinogen.
KW - Charge transfer resistance
KW - Electrochemical biosensor
KW - Electrochemical impedance spectroscopy
KW - Erythrocyte membrane
KW - Fibrinogen
UR - http://www.scopus.com/inward/record.url?scp=85065540459&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.05.016
DO - 10.1016/j.snb.2019.05.016
M3 - Article
AN - SCOPUS:85065540459
VL - 293
SP - 296
EP - 303
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -
