Ultrastable-Stealth Large Gold Nanoparticles with DNA Directed Biological Functionality

Jun Hyuk Heo, Kyung Il Kim, Hui Hun Cho, Jin Woong Lee, Byoung Sang Lee, Seokyoung Yoon, Kyung Jin Park, Seungwoo Lee, Jaeyun Kim, Dongmok Whang, Jung Heon Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The stability of gold nanoparticles (AuNPs) in biological samples is very important for their biomedical applications. Although various molecules such as polystyrenesulfonate (PSS), phosphine, DNA, and polyethylene glycol (PEG) have been used to stabilize AuNPs, it is still very difficult to stabilize large AuNPs. As a result, biomedical applications of large (30-100 nm) AuNPs are limited, even though they possess more favorable optical properties and are easier to be taken up by cells than smaller AuNPs. To overcome this limitation, we herein report a novel method of preparing large (30-100 nm) AuNPs with a high colloidal stability and facile chemical or biological functionality, via surface passivation with an amphiphilic polymer polyvinylpyrrolidone (PVP). This PVP passivation results in an extraordinary colloidal stability for 13, 30, 50, 70, and 100 nm AuNPs to be stabilized in PBS for at least 3 months. More importantly, the PVP capped AuNPs (AuNP-PVP) were also resistant to protein adsorption in the presence of serum containing media and exhibit a negligible cytotoxicity. The AuNP-PVPs functionalized with a DNA aptamer AS1411 remain biologically active, resulting in significant increase in the uptake of the AuNPs (∼12 200 AuNPs per cell) in comparison with AuNPs capped by a control DNA of the same length. The novel method developed in this study to stabilize large AuNPs with high colloidal stability and biological activity will allow much wider applications of these large AuNPs for biomedical applications, such as cellular imaging, molecular diagnosis, and targeted therapy.

Original languageEnglish
Pages (from-to)13773-13782
Number of pages10
JournalLangmuir
Volume31
Issue number51
DOIs
Publication statusPublished - 2015 Dec 29
Externally publishedYes

Fingerprint

Povidone
Gold
Nanoparticles
DNA
deoxyribonucleic acid
gold
nanoparticles
phosphine
Passivation
passivity
Nucleotide Aptamers
Molecular Targeted Therapy
Molecular imaging
Cytotoxicity
activity (biology)
Bioactivity
cells
phosphines
serums
Polyethylene glycols

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Heo, J. H., Kim, K. I., Cho, H. H., Lee, J. W., Lee, B. S., Yoon, S., ... Lee, J. H. (2015). Ultrastable-Stealth Large Gold Nanoparticles with DNA Directed Biological Functionality. Langmuir, 31(51), 13773-13782. https://doi.org/10.1021/acs.langmuir.5b03534

Ultrastable-Stealth Large Gold Nanoparticles with DNA Directed Biological Functionality. / Heo, Jun Hyuk; Kim, Kyung Il; Cho, Hui Hun; Lee, Jin Woong; Lee, Byoung Sang; Yoon, Seokyoung; Park, Kyung Jin; Lee, Seungwoo; Kim, Jaeyun; Whang, Dongmok; Lee, Jung Heon.

In: Langmuir, Vol. 31, No. 51, 29.12.2015, p. 13773-13782.

Research output: Contribution to journalArticle

Heo, JH, Kim, KI, Cho, HH, Lee, JW, Lee, BS, Yoon, S, Park, KJ, Lee, S, Kim, J, Whang, D & Lee, JH 2015, 'Ultrastable-Stealth Large Gold Nanoparticles with DNA Directed Biological Functionality', Langmuir, vol. 31, no. 51, pp. 13773-13782. https://doi.org/10.1021/acs.langmuir.5b03534
Heo, Jun Hyuk ; Kim, Kyung Il ; Cho, Hui Hun ; Lee, Jin Woong ; Lee, Byoung Sang ; Yoon, Seokyoung ; Park, Kyung Jin ; Lee, Seungwoo ; Kim, Jaeyun ; Whang, Dongmok ; Lee, Jung Heon. / Ultrastable-Stealth Large Gold Nanoparticles with DNA Directed Biological Functionality. In: Langmuir. 2015 ; Vol. 31, No. 51. pp. 13773-13782.
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