Ultrastructural comparison of porcine putative embryonic stem cells derived by in vitro fertilization and somatic cell nuclear transfer

Hyunju Yoo, Eunhye Kim, Seon Ung Hwang, Junchul David Yoon, Yubyeol Jeon, Kyu Mi Park, Kyu Jun Kim, Minghui Jin, Chang Kyu Lee, Eunsong Lee, Hyunggee Kim, Gonhyung Kim, Sang Hwan Hyun

Research output: Contribution to journalArticle

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Abstract

The ultrastructure of porcine putative embryonic stem cells and porcine fetal fibroblasts (PFFs) was analyzed by transmission electron microscopy. The aim of this study was to compare the features of organelles in in vitro fertilization (IVF) derived porcine embryonic stem cells (IVF-pESCs) and somatic cell nuclear transfer (SCNT) derived pESCs (SCNT-pESCs). Also, the features of organelles in High-Passage IVF-pESCs were compared with those in Low-Passage cells. The ultrastructure of PFFs showed rare microvilli on the cell surfaces, polygonal or irregular nuclei with one to two Reticular-Shaped nucleoli and euchromatin, low Cytoplasm-To-Nucleus ratios, rare ribosomes, rare rough endoplasmic reticulum, elongated mitochondria, rich lysosomes and rich phagocytic vacuoles. IVF-PESCs showed rare microvilli on the cell surfaces, round or irregular nuclei with one to two Reticular-Shaped nucleoli and euchromatin, low Cytoplasm-To-Nucleus ratios, rich ribosomes, long stacks of rough endoplasmic reticulum, elongated mitochondria, rare lysosomes and rare autophagic vacuoles. By contrast, SCNT-pESCs showed rich microvilli with various lengths and frequencies on the cell surfaces, polygonal nuclei with one reticular shaped nucleoli and heterochromatin, high Cytoplasm-To-Nucleus ratios, rare ribosomes, rare rough endoplasmic reticulum, round mitochondria, rich lysosomes and rich phagocytic vacuoles with clear intercellular junctions. Furthermore, High-Passage IVF-PESCs showed irregularly shaped colonies, pyknosis and numerous lysosomes associated with autophagic vacuoles showing signs of apoptosis. In conclusion, this study confirms that the ultrastructural characteristics of pESCs differ depending on their origin. These ultrastructural characteristics might be useful in biomedical research using pESCs, leading to new insights regarding regenerative medicine and tissue repair.

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalJournal of Reproduction and Development
Volume62
Issue number2
DOIs
Publication statusPublished - 2016 Apr 22

Fingerprint

embryonic stem cells
in vitro fertilization
lysosomes
somatic cells
vacuoles
rough endoplasmic reticulum
cell nucleolus
ribosomes
microvilli
swine
mitochondria
cytoplasm
fibroblasts
organelles
ultrastructure
cells
intercellular junctions
biomedical research
heterochromatin
tissue repair

Keywords

  • Embryonic stem cell
  • Porcine
  • Transmission electron microscopy
  • Ultrastructure

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Ultrastructural comparison of porcine putative embryonic stem cells derived by in vitro fertilization and somatic cell nuclear transfer. / Yoo, Hyunju; Kim, Eunhye; Hwang, Seon Ung; Yoon, Junchul David; Jeon, Yubyeol; Park, Kyu Mi; Kim, Kyu Jun; Jin, Minghui; Lee, Chang Kyu; Lee, Eunsong; Kim, Hyunggee; Kim, Gonhyung; Hyun, Sang Hwan.

In: Journal of Reproduction and Development, Vol. 62, No. 2, 22.04.2016, p. 177-185.

Research output: Contribution to journalArticle

Yoo, H, Kim, E, Hwang, SU, Yoon, JD, Jeon, Y, Park, KM, Kim, KJ, Jin, M, Lee, CK, Lee, E, Kim, H, Kim, G & Hyun, SH 2016, 'Ultrastructural comparison of porcine putative embryonic stem cells derived by in vitro fertilization and somatic cell nuclear transfer', Journal of Reproduction and Development, vol. 62, no. 2, pp. 177-185. https://doi.org/10.1262/jrd.2015-124
Yoo, Hyunju ; Kim, Eunhye ; Hwang, Seon Ung ; Yoon, Junchul David ; Jeon, Yubyeol ; Park, Kyu Mi ; Kim, Kyu Jun ; Jin, Minghui ; Lee, Chang Kyu ; Lee, Eunsong ; Kim, Hyunggee ; Kim, Gonhyung ; Hyun, Sang Hwan. / Ultrastructural comparison of porcine putative embryonic stem cells derived by in vitro fertilization and somatic cell nuclear transfer. In: Journal of Reproduction and Development. 2016 ; Vol. 62, No. 2. pp. 177-185.
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