Unaltered phenotype, tissue distribution and function of Vα14+ NKT cells in germ-free mice

Se Ho Park, Kamel Benlagha, Daniel Lee, Edward Balish, Albert Bendelac

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

The expression pattern of mouse CD1d and the tissue distribution of CD1d-restricted Vα14-Jα281 NKT cells suggest that the liver and the marginal zone of the spleen might be preferred sites of activation of this potent innate pathway of early cytokine secretion. Because these tissues are particularly involved with the filtration of blood-borne pathogens, and because NKT cells with an activated/memory phenotype accumulate over the first weeks of life and their CD1 ligands bind microbial glycolipids, it has been hypothesized that expansion of the NKT cell subset may be driven by exposure to the microbial environment. To test this hypothesis, we analyzed the frequency, surface phenotype and functional properties of NKT cells in normal and in germ-free C57BL/6 mice. Surprisingly, we found that the NKT cell subset develops in the presence or absence of a microbial environment. Although these results do not rule out the possibility that NKT cells exert a protective function against some microbial agents, they demonstrate that non microbial ligands, possibly self-antigens are sufficient for the generation, maturation and peripheral accumulation of NKT cells.

Original languageEnglish
Pages (from-to)620-625
Number of pages6
JournalEuropean Journal of Immunology
Volume30
Issue number2
DOIs
Publication statusPublished - 2000

Keywords

  • CD1
  • Infectious immunity
  • Repertoire development
  • T lymphocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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