Understanding β-mannanase from Streptomyces sp. CS147 and its potential application in lignocellulose based biorefining

Hah Y. Yoo, G. C. Pradeep, Soo K. Lee, Don H. Park, Seung S. Cho, Yun H. Choi, Jin C. Yoo, Seung W. Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hydrolytic enzymes such as cellulase and hemicellulase have been attracted in lignocellulose based biorefinery. Especially, mannanase has been a growing interest in industrial applications due to its importance in the bioconversion. In this study, an extracellular endo-β-1,4-D-mannanase was produced by Streptomyces sp. CS147 (Mn147) and purified 8.5-fold with a 43.4% yield using Sephadex G-50 column. The characterization of Mn147 was performed, and the results were as follows: molecular weight of ∼25 kDa with an optimum temperature of 50°C and pH of 11.0. The effect of metal ions and various reagents on Mn147 was strongly activated by Ca+2 but inhibited by Mg+2 , Fe+2 , hydrogen peroxide, EDTA and EGTA. Km and Vmax values of Mn147 were 0.13 mg/mL and 294 μmol/min mg, respectively, when different concentrations (3.1 to 50 mg/mL) of locust bean gum galactomannan were used as substrate. In enzymatic hydrolysis of heterogeneous substrate (spent coffee grounds), Mn147 shows a similar conversion compared to commercial enzymes. In addition, lignocellulosic biomass can be hydrolyzed to oligosaccharides (reducing sugars), which can be further utilized for the production of biomaterials. These results showed that Mn147 is attractive in quest of potential bioindustrial applications.

Original languageEnglish
Pages (from-to)1894-1902
Number of pages9
JournalBiotechnology Journal
Volume10
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

Keywords

  • Biomass
  • Biorefinery
  • Lignocellulose
  • Streptomyces
  • β-mannanase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

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