Understanding the Effects of Genotype, Growing Year, and Breeding on Tunisian Durum Wheat Allergenicity. 1. the Baker's Asthma Case

Fatma Boukid, Barbara Prandi, Stefano Sforza, Rhouma Sayar, Yong Weon Seo, Mondher Mejri, Ines Yacoubi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Baker's asthma is a serious airway disease triggered by wheat protein CM3 α-amylase/trypsin inhibitor. The purpose of the present study was to investigate the impact of genotype and crop year on allergen CM3 α-amylase/trypsin inhibitor associated with baker's asthma. A historical series of Tunisian durum wheat (100 accessions), derived from three crop years, was used to compare the amount of CM3 from landraces to advanced cultivars. CM3 protein quantification was assessed after an enzymatic cleavage of the soluble protein extracts on a UPLC/ESI-MS system, using a marker peptide for its quantification. Combined data analysis of variance revealed an important effect of genotype, crop year, and their interaction. The CM3 allergenic proteins were found to significantly vary among studied genotypes, as confirmed by genetic variability, coefficient of variance, heritability, and genetic advance.

Original languageEnglish
Pages (from-to)5831-5836
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number28
DOIs
Publication statusPublished - 2017 Jul 19

Fingerprint

allergenicity
crop year
asthma
durum wheat
Triticum
Breeding
Asthma
Genotype
trypsin inhibitors
Crops
amylases
Trypsin Inhibitors
genotype
breeding
Amylases
Proteins
wheat protein
proteins
allergens
landraces

Keywords

  • allergenicity
  • baker's asthma
  • CM3 α-amylase/trypsin inhibitor
  • durum wheat

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Understanding the Effects of Genotype, Growing Year, and Breeding on Tunisian Durum Wheat Allergenicity. 1. the Baker's Asthma Case. / Boukid, Fatma; Prandi, Barbara; Sforza, Stefano; Sayar, Rhouma; Seo, Yong Weon; Mejri, Mondher; Yacoubi, Ines.

In: Journal of Agricultural and Food Chemistry, Vol. 65, No. 28, 19.07.2017, p. 5831-5836.

Research output: Contribution to journalArticle

Boukid, Fatma ; Prandi, Barbara ; Sforza, Stefano ; Sayar, Rhouma ; Seo, Yong Weon ; Mejri, Mondher ; Yacoubi, Ines. / Understanding the Effects of Genotype, Growing Year, and Breeding on Tunisian Durum Wheat Allergenicity. 1. the Baker's Asthma Case. In: Journal of Agricultural and Food Chemistry. 2017 ; Vol. 65, No. 28. pp. 5831-5836.
@article{f757a187d2294b189a94047289c58332,
title = "Understanding the Effects of Genotype, Growing Year, and Breeding on Tunisian Durum Wheat Allergenicity. 1. the Baker's Asthma Case",
abstract = "Baker's asthma is a serious airway disease triggered by wheat protein CM3 α-amylase/trypsin inhibitor. The purpose of the present study was to investigate the impact of genotype and crop year on allergen CM3 α-amylase/trypsin inhibitor associated with baker's asthma. A historical series of Tunisian durum wheat (100 accessions), derived from three crop years, was used to compare the amount of CM3 from landraces to advanced cultivars. CM3 protein quantification was assessed after an enzymatic cleavage of the soluble protein extracts on a UPLC/ESI-MS system, using a marker peptide for its quantification. Combined data analysis of variance revealed an important effect of genotype, crop year, and their interaction. The CM3 allergenic proteins were found to significantly vary among studied genotypes, as confirmed by genetic variability, coefficient of variance, heritability, and genetic advance.",
keywords = "allergenicity, baker's asthma, CM3 α-amylase/trypsin inhibitor, durum wheat",
author = "Fatma Boukid and Barbara Prandi and Stefano Sforza and Rhouma Sayar and Seo, {Yong Weon} and Mondher Mejri and Ines Yacoubi",
year = "2017",
month = "7",
day = "19",
doi = "10.1021/acs.jafc.7b02040",
language = "English",
volume = "65",
pages = "5831--5836",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "28",

}

TY - JOUR

T1 - Understanding the Effects of Genotype, Growing Year, and Breeding on Tunisian Durum Wheat Allergenicity. 1. the Baker's Asthma Case

AU - Boukid, Fatma

AU - Prandi, Barbara

AU - Sforza, Stefano

AU - Sayar, Rhouma

AU - Seo, Yong Weon

AU - Mejri, Mondher

AU - Yacoubi, Ines

PY - 2017/7/19

Y1 - 2017/7/19

N2 - Baker's asthma is a serious airway disease triggered by wheat protein CM3 α-amylase/trypsin inhibitor. The purpose of the present study was to investigate the impact of genotype and crop year on allergen CM3 α-amylase/trypsin inhibitor associated with baker's asthma. A historical series of Tunisian durum wheat (100 accessions), derived from three crop years, was used to compare the amount of CM3 from landraces to advanced cultivars. CM3 protein quantification was assessed after an enzymatic cleavage of the soluble protein extracts on a UPLC/ESI-MS system, using a marker peptide for its quantification. Combined data analysis of variance revealed an important effect of genotype, crop year, and their interaction. The CM3 allergenic proteins were found to significantly vary among studied genotypes, as confirmed by genetic variability, coefficient of variance, heritability, and genetic advance.

AB - Baker's asthma is a serious airway disease triggered by wheat protein CM3 α-amylase/trypsin inhibitor. The purpose of the present study was to investigate the impact of genotype and crop year on allergen CM3 α-amylase/trypsin inhibitor associated with baker's asthma. A historical series of Tunisian durum wheat (100 accessions), derived from three crop years, was used to compare the amount of CM3 from landraces to advanced cultivars. CM3 protein quantification was assessed after an enzymatic cleavage of the soluble protein extracts on a UPLC/ESI-MS system, using a marker peptide for its quantification. Combined data analysis of variance revealed an important effect of genotype, crop year, and their interaction. The CM3 allergenic proteins were found to significantly vary among studied genotypes, as confirmed by genetic variability, coefficient of variance, heritability, and genetic advance.

KW - allergenicity

KW - baker's asthma

KW - CM3 α-amylase/trypsin inhibitor

KW - durum wheat

UR - http://www.scopus.com/inward/record.url?scp=85025072985&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85025072985&partnerID=8YFLogxK

U2 - 10.1021/acs.jafc.7b02040

DO - 10.1021/acs.jafc.7b02040

M3 - Article

C2 - 28644028

AN - SCOPUS:85025072985

VL - 65

SP - 5831

EP - 5836

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 28

ER -