Undetactable levels of genotoxicity of SiO2 nanoparticles in in vitro and in vivo tests

Jee Young Kwon, Hye Lim Kim, Jong Yun Lee, Yo Han Ju, Ji Soo Kim, Seung Hun Kang, Yu Ri Kim, Jong Kwon Lee, Jayoung Jeong, Meyoung-Kon Kim, Eun Ho Maeng, Young Rok Seo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Silica dioxide (SiO2) has been used in various industrial products, including paints and coatings, plastics, synthetic rubbers, and adhesives. Several studies have investigated the genotoxic effects of SiO2; however, the results remain controversial due to variations in the evaluation methods applied in determining its physicochemical properties. Thus, well characterized chemicals and standardized methods are needed for better assessment of the genotoxicity of nanoparticles. Methods: The genotoxicity of SiO2 was evaluated using two types of well characterized SiO2, ie, 20 nm (-) charge (SiO2 EN20(-)) and 100 nm (-) charge (SiO2 EN100(-)). Four end point genotoxicity tests, ie, the bacterial mutation assay, in vitro chromosomal aberration test, in vivo comet assay, and in vivo micronucleus test, were conducted following the test guidelines of the Organization for Economic Cooperation and Development (OECD) with application of Good Laboratory Practice. Results: No statistically significant differences were found in the bacterial mutation assay, in vitro chromosomal aberration test, in vivo comet assay, and in vivo micronucleus test when tested for induction of genotoxicity in both two types of SiO2 nanoparticles. Conclusion: These results suggest that SiO2 nanoparticles, in particular SiO2 EN20(-) and SiO2EN100(-), are not genotoxic in both in vitro and in vivo systems under OECD guidelines. Further, the results were generated in accordance with OECD test guidelines, and Good Laboratory Practice application; it can be accepted as reliable information regarding SiO2-induced genotoxicity.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalInternational Journal of Nanomedicine
Volume9
DOIs
Publication statusPublished - 2014 Dec 15

Fingerprint

Nanoparticles
Assays
Micronucleus Tests
Comet Assay
Guidelines
Chromosome Aberrations
Aberrations
Elastomers
Mutagenicity Tests
Economics
Mutation
Paint
Plastic coatings
Synthetic rubber
Silicon Dioxide
Adhesives
Plastics
Silica
In Vitro Techniques
Organisation for Economic Co-Operation and Development

Keywords

  • Genotoxicity test
  • Good Laboratory Practice
  • Organization for Economic Cooperation and Development test guideline
  • Silica dioxide

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Kwon, J. Y., Kim, H. L., Lee, J. Y., Ju, Y. H., Kim, J. S., Kang, S. H., ... Seo, Y. R. (2014). Undetactable levels of genotoxicity of SiO2 nanoparticles in in vitro and in vivo tests. International Journal of Nanomedicine, 9, 173-181. https://doi.org/10.2147/IJN.S57933

Undetactable levels of genotoxicity of SiO2 nanoparticles in in vitro and in vivo tests. / Kwon, Jee Young; Kim, Hye Lim; Lee, Jong Yun; Ju, Yo Han; Kim, Ji Soo; Kang, Seung Hun; Kim, Yu Ri; Lee, Jong Kwon; Jeong, Jayoung; Kim, Meyoung-Kon; Maeng, Eun Ho; Seo, Young Rok.

In: International Journal of Nanomedicine, Vol. 9, 15.12.2014, p. 173-181.

Research output: Contribution to journalArticle

Kwon, JY, Kim, HL, Lee, JY, Ju, YH, Kim, JS, Kang, SH, Kim, YR, Lee, JK, Jeong, J, Kim, M-K, Maeng, EH & Seo, YR 2014, 'Undetactable levels of genotoxicity of SiO2 nanoparticles in in vitro and in vivo tests', International Journal of Nanomedicine, vol. 9, pp. 173-181. https://doi.org/10.2147/IJN.S57933
Kwon, Jee Young ; Kim, Hye Lim ; Lee, Jong Yun ; Ju, Yo Han ; Kim, Ji Soo ; Kang, Seung Hun ; Kim, Yu Ri ; Lee, Jong Kwon ; Jeong, Jayoung ; Kim, Meyoung-Kon ; Maeng, Eun Ho ; Seo, Young Rok. / Undetactable levels of genotoxicity of SiO2 nanoparticles in in vitro and in vivo tests. In: International Journal of Nanomedicine. 2014 ; Vol. 9. pp. 173-181.
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