Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays

Jong Min Lee, Joo Yoon Moon, Mohammed R. Shaker, Woong Sun, Bong Geun Chung

Research output: Contribution to journalArticle

1 Citation (Scopus)


We developed the photocrosslinkable hydrogel microwell arrays for uniform-sized neurosphere-mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform-sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform-sized neurospheres harvested from microwell arrays were replated into laminin-coated substrate. In parallel, uniform-sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three-dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform-sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform-sized neurosphere-mediated motoneuron differentiation.

Original languageEnglish
Publication statusAccepted/In press - 2017


  • Hydrogel microwell array
  • Motoneuron differentiation
  • Uniform-sized neurosphere

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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