Unique properties of four Lactobacilli in amino acid production and symbiotic mixed culture for lactic acid biosynthesis

Kibeom Lee, Jeewon Lee, Yang Hoon Kim, Seung Hyeon Moon, Young Hoon Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

With four Lactobacilli - L. delbrueckii subsp. lactis (ATCC 12315), L. casei (NRRL-B1445), L. delbrueckii (NRRL-B445), and L. heveticus (NRRL-B1937) - the characteristics of cell growth and production of lactate and amino acids were investigated. Especially, the time-course variation in concentration of amino acids (classified into alanine, serine, aspartate, glutamate, aromatic amino acid, and histidine families) was estimated in detail, and the results were systematically compared. It was elucidated that L. delbrueckii (NRRL-B445) and L. helveticus (NRRL-B1937) had quite different characteristics in growth, lactic acid synthesis, and amino acid production. L. helveticus (NRRL-B1937) was superior in the production of amino acids as well as in cell growth, but showed very poor ability in lactic acid production. However, L. delbrueckii (NRRL-B445) showed higher yield of lactic acid despite repressed cell growth, but suffered from severe amino acid deficiency in culture. By modulating the initial concentration of each strain in the mixed culture containing both L. delbrueckii (NRRL-B445) and L. helveticus (NRRL-B1937), the lactic acid production (i.e., the amount of lactic acid produced and lactic acid yield to glucose consumed) was significantly improved, presumably via symbiotic interaction between the two strains.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalCurrent Microbiology
Volume43
Issue number6
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Lactobacillus
Lactic Acid
Amino Acids
Growth
Lactobacillus delbrueckii
Aromatic Amino Acids
Histidine
Aspartic Acid
Alanine
Serine
Glutamic Acid
Glucose

ASJC Scopus subject areas

  • Microbiology

Cite this

Unique properties of four Lactobacilli in amino acid production and symbiotic mixed culture for lactic acid biosynthesis. / Lee, Kibeom; Lee, Jeewon; Kim, Yang Hoon; Moon, Seung Hyeon; Park, Young Hoon.

In: Current Microbiology, Vol. 43, No. 6, 2001, p. 383-390.

Research output: Contribution to journalArticle

Lee, Kibeom ; Lee, Jeewon ; Kim, Yang Hoon ; Moon, Seung Hyeon ; Park, Young Hoon. / Unique properties of four Lactobacilli in amino acid production and symbiotic mixed culture for lactic acid biosynthesis. In: Current Microbiology. 2001 ; Vol. 43, No. 6. pp. 383-390.
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