Abstract
Objectives: To investigate the expression of peroxisome proliferator-activated receptor γ (PPAR-γ) messenger RNA and protein and to localize the PPAR-γ protein in the nasal mucosa of patients with allergic rhinitis and control subjects. Design: Prospective study. Setting: Tertiary academic institution. Patients: Twenty patients with perennial allergic rhinitis and 20 matched nonallergic patients. Interventions: Inferior turbinate mucosa samples were obtained from 20 patients with perennial allergic rhinitis and 20 matched nonallegic patients. Peroxisome proliferator-activated receptor γ messenger RNA was extracted from the inferior turbinate mucosae, and then reverse transcription-polymerase chain reaction was performed. Western blot testing was used to analyze differences in PPAR-γ protein expression levels between patients with allergic rhinitis and normal controls, and the PPAR-γ protein was localized immunohistochemically. Results: The expression levels of PPAR-γ messenger RNA and protein in the nasal mucosa was significantly increased in patients with perennial allergic rhinitis compared with controls. Peroxisome proliferator-activated receptor γ protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands. Conclusions: Peroxisome proliferator-activated receptor γ is expressed in the human nasal mucosa and is up-regulated in perennial allergic rhinitis. These results suggest a possible contribution for PPAR-γ in chronic inflammation of the nasal mucosa in perennial allergic rhinitis.
| Original language | English |
|---|---|
| Pages (from-to) | 1196-1200 |
| Number of pages | 5 |
| Journal | Archives of Otolaryngology - Head and Neck Surgery |
| Volume | 132 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2006 Nov 28 |
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ASJC Scopus subject areas
- Otorhinolaryngology
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Up-regulation of peroxisome proliferator-activated receptor γ in perennial allergic rhinitis. / Hee, Joon Kang; Cinn, Young Gi; Soon, Jae Hwang; Chae, Sungwon; Woo, Jeong-Soo; Lee, Sang Hag; Lee, Heung Man.
In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 132, No. 11, 28.11.2006, p. 1196-1200.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Up-regulation of peroxisome proliferator-activated receptor γ in perennial allergic rhinitis
AU - Hee, Joon Kang
AU - Cinn, Young Gi
AU - Soon, Jae Hwang
AU - Chae, Sungwon
AU - Woo, Jeong-Soo
AU - Lee, Sang Hag
AU - Lee, Heung Man
PY - 2006/11/28
Y1 - 2006/11/28
N2 - Objectives: To investigate the expression of peroxisome proliferator-activated receptor γ (PPAR-γ) messenger RNA and protein and to localize the PPAR-γ protein in the nasal mucosa of patients with allergic rhinitis and control subjects. Design: Prospective study. Setting: Tertiary academic institution. Patients: Twenty patients with perennial allergic rhinitis and 20 matched nonallergic patients. Interventions: Inferior turbinate mucosa samples were obtained from 20 patients with perennial allergic rhinitis and 20 matched nonallegic patients. Peroxisome proliferator-activated receptor γ messenger RNA was extracted from the inferior turbinate mucosae, and then reverse transcription-polymerase chain reaction was performed. Western blot testing was used to analyze differences in PPAR-γ protein expression levels between patients with allergic rhinitis and normal controls, and the PPAR-γ protein was localized immunohistochemically. Results: The expression levels of PPAR-γ messenger RNA and protein in the nasal mucosa was significantly increased in patients with perennial allergic rhinitis compared with controls. Peroxisome proliferator-activated receptor γ protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands. Conclusions: Peroxisome proliferator-activated receptor γ is expressed in the human nasal mucosa and is up-regulated in perennial allergic rhinitis. These results suggest a possible contribution for PPAR-γ in chronic inflammation of the nasal mucosa in perennial allergic rhinitis.
AB - Objectives: To investigate the expression of peroxisome proliferator-activated receptor γ (PPAR-γ) messenger RNA and protein and to localize the PPAR-γ protein in the nasal mucosa of patients with allergic rhinitis and control subjects. Design: Prospective study. Setting: Tertiary academic institution. Patients: Twenty patients with perennial allergic rhinitis and 20 matched nonallergic patients. Interventions: Inferior turbinate mucosa samples were obtained from 20 patients with perennial allergic rhinitis and 20 matched nonallegic patients. Peroxisome proliferator-activated receptor γ messenger RNA was extracted from the inferior turbinate mucosae, and then reverse transcription-polymerase chain reaction was performed. Western blot testing was used to analyze differences in PPAR-γ protein expression levels between patients with allergic rhinitis and normal controls, and the PPAR-γ protein was localized immunohistochemically. Results: The expression levels of PPAR-γ messenger RNA and protein in the nasal mucosa was significantly increased in patients with perennial allergic rhinitis compared with controls. Peroxisome proliferator-activated receptor γ protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands. Conclusions: Peroxisome proliferator-activated receptor γ is expressed in the human nasal mucosa and is up-regulated in perennial allergic rhinitis. These results suggest a possible contribution for PPAR-γ in chronic inflammation of the nasal mucosa in perennial allergic rhinitis.
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UR - http://www.scopus.com/inward/citedby.url?scp=33751278717&partnerID=8YFLogxK
U2 - 10.1001/archotol.132.11.1196
DO - 10.1001/archotol.132.11.1196
M3 - Article
C2 - 17116814
AN - SCOPUS:33751278717
VL - 132
SP - 1196
EP - 1200
JO - JAMA Otolaryngology - Head and Neck Surgery
JF - JAMA Otolaryngology - Head and Neck Surgery
SN - 2168-6181
IS - 11
ER -