Abstract
Objectives: We compared the patterns of PAR-2 messenger RNA (mRNA) and protein expression in the nasal mucosa of subjects with and without allergic rhinitis. Methods: Biopsy specimens were obtained from 10 patients with allergic rhinitis and 10 normal controls. RNA was extracted from the nasal mucosa, and semiquantitative reverse transcription-polymerase chain reaction was performed for PAR-2. Tissue sections were immunostained for PAR-2 by use of specific antibody. Results: The expression levels of PAR-2 mRNA in allergic rhinitis nasal mucosa were significantly up-regulated as compared with those in normal nasal mucosa. PAR-2 immunoreactivity was observed in the epithelium and submucosal glands in both normal controls and subjects with allergic rhinitis. Stronger immunoreactivity for PAR-2 was observed in allergic rhinitis nasal mucosa as compared with normal nasal mucosa. Conclusions: These results suggest that PAR-2 may be involved in allergic nasal inflammation.
| Original language | English |
|---|---|
| Pages (from-to) | 554-558 |
| Number of pages | 5 |
| Journal | Annals of Otology, Rhinology and Laryngology |
| Volume | 116 |
| Issue number | 7 |
| Publication status | Published - 2007 Jul 1 |
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Keywords
- Allergic rhinitis
- Protease-activated receptor
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Up-regulation of protease-activated receptor 2 in allergic rhinitis. / Lee, Heung Man; Hyo, Yeol Kim; Hee, Joon Kang; Woo, Jeong-Soo; Chae, Sungwon; Lee, Sang Hag; Soon, Jae Hwang.
In: Annals of Otology, Rhinology and Laryngology, Vol. 116, No. 7, 01.07.2007, p. 554-558.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Up-regulation of protease-activated receptor 2 in allergic rhinitis
AU - Lee, Heung Man
AU - Hyo, Yeol Kim
AU - Hee, Joon Kang
AU - Woo, Jeong-Soo
AU - Chae, Sungwon
AU - Lee, Sang Hag
AU - Soon, Jae Hwang
PY - 2007/7/1
Y1 - 2007/7/1
N2 - Objectives: We compared the patterns of PAR-2 messenger RNA (mRNA) and protein expression in the nasal mucosa of subjects with and without allergic rhinitis. Methods: Biopsy specimens were obtained from 10 patients with allergic rhinitis and 10 normal controls. RNA was extracted from the nasal mucosa, and semiquantitative reverse transcription-polymerase chain reaction was performed for PAR-2. Tissue sections were immunostained for PAR-2 by use of specific antibody. Results: The expression levels of PAR-2 mRNA in allergic rhinitis nasal mucosa were significantly up-regulated as compared with those in normal nasal mucosa. PAR-2 immunoreactivity was observed in the epithelium and submucosal glands in both normal controls and subjects with allergic rhinitis. Stronger immunoreactivity for PAR-2 was observed in allergic rhinitis nasal mucosa as compared with normal nasal mucosa. Conclusions: These results suggest that PAR-2 may be involved in allergic nasal inflammation.
AB - Objectives: We compared the patterns of PAR-2 messenger RNA (mRNA) and protein expression in the nasal mucosa of subjects with and without allergic rhinitis. Methods: Biopsy specimens were obtained from 10 patients with allergic rhinitis and 10 normal controls. RNA was extracted from the nasal mucosa, and semiquantitative reverse transcription-polymerase chain reaction was performed for PAR-2. Tissue sections were immunostained for PAR-2 by use of specific antibody. Results: The expression levels of PAR-2 mRNA in allergic rhinitis nasal mucosa were significantly up-regulated as compared with those in normal nasal mucosa. PAR-2 immunoreactivity was observed in the epithelium and submucosal glands in both normal controls and subjects with allergic rhinitis. Stronger immunoreactivity for PAR-2 was observed in allergic rhinitis nasal mucosa as compared with normal nasal mucosa. Conclusions: These results suggest that PAR-2 may be involved in allergic nasal inflammation.
KW - Allergic rhinitis
KW - Protease-activated receptor
UR - http://www.scopus.com/inward/record.url?scp=34447509746&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34447509746&partnerID=8YFLogxK
M3 - Article
VL - 116
SP - 554
EP - 558
JO - Annals of Otology, Rhinology and Laryngology
JF - Annals of Otology, Rhinology and Laryngology
SN - 0003-4894
IS - 7
ER -