Upregulation of class II β-tubulin expression in differentiating keratinocytes

Woong Hee Lee, Joo Young Kim, Young Sik Kim, Hye Joon Song, Ki Joon Song, Jin Won Song, Luck Ju Baek, Eun Young Seo, Chang Duk Kim, Jeung Hoon Lee, Sun Ho Kee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The diverse functions of microtubules (MT) in different cells and tissues may be facilitated by compositional changes in tubulin isotypes. We obtained partial cDNA clones of class II β-tubulin from a library of differentiating normal human epidermal keratinocytes (NHEK) cells, whereas screening via subtractive hybridization for genes involved in calcium-induced keratinocyte differentiation. Analysis of the isotypic composition of β-tubulin from NHEK cells revealed elevations in class II β-tubulin concentrations at both protein and message levels during cell differentiation, resulting in increased rates of incorporation of class II β-tubulin into MT. Immunohistochemistry of normal and pathologic skin tissues showed that class II β-tubulin occurred in the granular layer of the epidermis and in differentiated areas of carcinomas. Class II β-tubulin was, however, not observed in the uppermost granular and cornified layers of normal epidermis. Further experiments showed that MT were likely to decay in the final stage of terminal differentiation during formation of the cornified envelope. Our results suggest that there is differential modulation of MT composition and stability during keratinocyte differentiation.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalJournal of Investigative Dermatology
Volume124
Issue number2
DOIs
Publication statusPublished - 2005 Feb

Keywords

  • Class II β-tubulin
  • Differentiation
  • Keratinocytes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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