Upregulation of the cysteine protease inhibitor, cystatin SN, contributes to cell proliferation and cathepsin inhibition in gastric cancer

Eun Hwa Choi, Jong Tae Kim, Joo Heon Kim, Soo Young Kim, Eun Young Song, Jae Wha Kim, Seon Young Kim, Young Il Yeom, Ik Hwan Kim, Hee Gu Lee

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Cysteine proteases like cathepsins are widely distributed proteolytic enzymes and form tight equimolar complexes with cystatins at their active sites. Among cystatins, CST1, encoding cystatin SN, is a member of the type 2 salivary cystatin family found in a variety of fluids and secretions, including plasma, tears, and saliva. CST1 was identified as an upregulated gene in gastric cancer tissues compared to noncancerous regions using our Affymetrix GeneChip microarray. Methods: The upregulation of CST1 in gastric cancer was analyzed using RT-PCR (n = 15), immnohistochemistry, and clinicopathological (n = 77) analysis. CST1-siRNA was used for the suppression of CST1 gene expression and cathepsin proteolytic activity was assayed. Results: CST1 was upregulated in cancerous lesions of gastric cancer tissues compared to noncancerous regions and clinicopathological analysis showed a significant correlation between high expression of CST1 and pTNM stage (p = 0.044). In CST1-siRNA transfected cells, cell proliferation was reduced and the proteolytic activity of cathepsins was increased. Conclusions: CST1 might be highly involved in gastric tumorigenesis and regulate the proteolytic activity of cysteine proteases. Crown

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalClinica Chimica Acta
Volume406
Issue number1-2
DOIs
Publication statusPublished - 2009 Aug 11

Fingerprint

Salivary Cystatins
Cystatins
Cysteine Proteinase Inhibitors
Cathepsins
Cell proliferation
Stomach Neoplasms
Up-Regulation
Cysteine Proteases
Cell Proliferation
Small Interfering RNA
Tissue
Fluids and Secretions
Microarrays
Crowns
Tears
Saliva
Gene expression
Catalytic Domain
Stomach
Carcinogenesis

Keywords

  • Cathepsin
  • CST1
  • Cystatin SN
  • Gastric cancer
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Upregulation of the cysteine protease inhibitor, cystatin SN, contributes to cell proliferation and cathepsin inhibition in gastric cancer. / Choi, Eun Hwa; Kim, Jong Tae; Kim, Joo Heon; Kim, Soo Young; Song, Eun Young; Kim, Jae Wha; Kim, Seon Young; Yeom, Young Il; Kim, Ik Hwan; Lee, Hee Gu.

In: Clinica Chimica Acta, Vol. 406, No. 1-2, 11.08.2009, p. 45-51.

Research output: Contribution to journalArticle

Choi, Eun Hwa ; Kim, Jong Tae ; Kim, Joo Heon ; Kim, Soo Young ; Song, Eun Young ; Kim, Jae Wha ; Kim, Seon Young ; Yeom, Young Il ; Kim, Ik Hwan ; Lee, Hee Gu. / Upregulation of the cysteine protease inhibitor, cystatin SN, contributes to cell proliferation and cathepsin inhibition in gastric cancer. In: Clinica Chimica Acta. 2009 ; Vol. 406, No. 1-2. pp. 45-51.
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