Upregulation of VEGF and FGF2 in normal rat brain after experimental intraoperative radiation therapy

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Abstract

The expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF)2 in the irradiated brain was examined to test how a single high dose radiation, similar to that used for intraoperative radiation therapy given to the normal cerebrum, can affect the vascular endothelium. After a burr hole trephination in the rat skull, the cerebral hemisphere was exposed to a single 10 Gy dose of gamma rays, and the radiation effect was assessed at 1, 2, 4, 6, and 8 weeks after irradiation. Histological changes, such as reactive gliosis, inflammation, vascular proliferation and necrosis, were correlated with the duration after irradiation. Significant VEGF and FGF2 expression in the 2- and 8-week were detected by enzyme-linked immunosorbent assay quantification in the radiation group. Immunohistochemical study for VEGF was done and the number of positive cells gradually increased over time, compared with the sham operation group. In conclusion, the radiation injuries consisted of radiation necrosis associated with the expression of VEGF and FGF2. These findings indicate that VEGF and FGF2 may play a role in the radiation injuries after intraoperative single high-dose irradiation.

Original languageEnglish
Pages (from-to)879-886
Number of pages8
JournalJournal of Korean Medical Science
Volume19
Issue number6
Publication statusPublished - 2004 Dec 1

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Fibroblast Growth Factor 2
Vascular Endothelial Growth Factor A
Up-Regulation
Radiotherapy
Brain
Radiation Injuries
Gamma Rays
Cerebrum
Radiation
Necrosis
Trephining
Gliosis
Radiation Effects
Vascular Endothelium
Skull
Blood Vessels
Cell Count
Enzyme-Linked Immunosorbent Assay
Inflammation

Keywords

  • Fibroblast growth factor 2
  • Radiation injuries, experimental
  • Radiotherapy
  • Vascular endothelial growth factor A

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Upregulation of VEGF and FGF2 in normal rat brain after experimental intraoperative radiation therapy",
abstract = "The expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF)2 in the irradiated brain was examined to test how a single high dose radiation, similar to that used for intraoperative radiation therapy given to the normal cerebrum, can affect the vascular endothelium. After a burr hole trephination in the rat skull, the cerebral hemisphere was exposed to a single 10 Gy dose of gamma rays, and the radiation effect was assessed at 1, 2, 4, 6, and 8 weeks after irradiation. Histological changes, such as reactive gliosis, inflammation, vascular proliferation and necrosis, were correlated with the duration after irradiation. Significant VEGF and FGF2 expression in the 2- and 8-week were detected by enzyme-linked immunosorbent assay quantification in the radiation group. Immunohistochemical study for VEGF was done and the number of positive cells gradually increased over time, compared with the sham operation group. In conclusion, the radiation injuries consisted of radiation necrosis associated with the expression of VEGF and FGF2. These findings indicate that VEGF and FGF2 may play a role in the radiation injuries after intraoperative single high-dose irradiation.",
keywords = "Fibroblast growth factor 2, Radiation injuries, experimental, Radiotherapy, Vascular endothelial growth factor A",
author = "Joo-Han Kim and Chung, {Yong Gu} and Kim, {Chul Yong} and Kim, {Han Kyeom} and Lee, {Hoon Kap}",
year = "2004",
month = "12",
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language = "English",
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T1 - Upregulation of VEGF and FGF2 in normal rat brain after experimental intraoperative radiation therapy

AU - Kim, Joo-Han

AU - Chung, Yong Gu

AU - Kim, Chul Yong

AU - Kim, Han Kyeom

AU - Lee, Hoon Kap

PY - 2004/12/1

Y1 - 2004/12/1

N2 - The expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF)2 in the irradiated brain was examined to test how a single high dose radiation, similar to that used for intraoperative radiation therapy given to the normal cerebrum, can affect the vascular endothelium. After a burr hole trephination in the rat skull, the cerebral hemisphere was exposed to a single 10 Gy dose of gamma rays, and the radiation effect was assessed at 1, 2, 4, 6, and 8 weeks after irradiation. Histological changes, such as reactive gliosis, inflammation, vascular proliferation and necrosis, were correlated with the duration after irradiation. Significant VEGF and FGF2 expression in the 2- and 8-week were detected by enzyme-linked immunosorbent assay quantification in the radiation group. Immunohistochemical study for VEGF was done and the number of positive cells gradually increased over time, compared with the sham operation group. In conclusion, the radiation injuries consisted of radiation necrosis associated with the expression of VEGF and FGF2. These findings indicate that VEGF and FGF2 may play a role in the radiation injuries after intraoperative single high-dose irradiation.

AB - The expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF)2 in the irradiated brain was examined to test how a single high dose radiation, similar to that used for intraoperative radiation therapy given to the normal cerebrum, can affect the vascular endothelium. After a burr hole trephination in the rat skull, the cerebral hemisphere was exposed to a single 10 Gy dose of gamma rays, and the radiation effect was assessed at 1, 2, 4, 6, and 8 weeks after irradiation. Histological changes, such as reactive gliosis, inflammation, vascular proliferation and necrosis, were correlated with the duration after irradiation. Significant VEGF and FGF2 expression in the 2- and 8-week were detected by enzyme-linked immunosorbent assay quantification in the radiation group. Immunohistochemical study for VEGF was done and the number of positive cells gradually increased over time, compared with the sham operation group. In conclusion, the radiation injuries consisted of radiation necrosis associated with the expression of VEGF and FGF2. These findings indicate that VEGF and FGF2 may play a role in the radiation injuries after intraoperative single high-dose irradiation.

KW - Fibroblast growth factor 2

KW - Radiation injuries, experimental

KW - Radiotherapy

KW - Vascular endothelial growth factor A

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