Use of immobilized phospholipase A1-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content

R. Baeza-Jiménez; J. González-Rodríguez; I. H. Kim; H. S. García; C. Otero

doi:10.3989/gya.045211

Use of immobilized phospholipase A₁-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content

R. Baeza-Jiménez, J. González-Rodríguez, I. H. Kim, H. S. García, C. Otero

Department of Public Health Sciences

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A₁ (PLA₁) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.

Original language	English
Pages (from-to)	44-52
Number of pages	9
Journal	Grasas y Aceites
Volume	63
Issue number	1
DOIs	https://doi.org/10.3989/gya.045211
Publication status	Published - 2012 Jan

Keywords

Acidolysis
Conjugated linoleic acid
Phosphatidylcholine
Phospholipase A
Structured phospholipids

ASJC Scopus subject areas

Food Science
Organic Chemistry

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Use of immobilized phospholipase A₁-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content. / Baeza-Jiménez, R.; González-Rodríguez, J.; Kim, I. H.; García, H. S.; Otero, C.

In: Grasas y Aceites, Vol. 63, No. 1, 01.2012, p. 44-52.

Research output: Contribution to journal › Article › peer-review

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