Use of immobilized phospholipase A1-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content

R. Baeza-Jiménez, J. González-Rodríguez, In-Hwan Kim, H. S. García, C. Otero

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A1 (PLA1) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.

Original languageEnglish
Pages (from-to)44-52
Number of pages9
JournalGrasas y Aceites
Volume63
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

phospholipase A1
Phospholipases A1
acidolysis
Conjugated Linoleic Acids
conjugated linoleic acid
Phosphatidylcholines
phosphatidylcholines
Substrates
Enzymes
enzymes
Weights and Measures
Temperature
temperature

Keywords

  • Acidolysis
  • Conjugated linoleic acid
  • Phosphatidylcholine
  • Phospholipase A
  • Structured phospholipids

ASJC Scopus subject areas

  • Food Science
  • Organic Chemistry

Cite this

Use of immobilized phospholipase A1-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content. / Baeza-Jiménez, R.; González-Rodríguez, J.; Kim, In-Hwan; García, H. S.; Otero, C.

In: Grasas y Aceites, Vol. 63, No. 1, 01.01.2012, p. 44-52.

Research output: Contribution to journalArticle

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AU - González-Rodríguez, J.

AU - Kim, In-Hwan

AU - García, H. S.

AU - Otero, C.

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