TY - JOUR
T1 - Use of immobilized phospholipase A1-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content
AU - Baeza-Jiménez, R.
AU - González-Rodríguez, J.
AU - Kim, I. H.
AU - García, H. S.
AU - Otero, C.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/1
Y1 - 2012/1
N2 - Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A1 (PLA1) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.
AB - Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A1 (PLA1) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.
KW - Acidolysis
KW - Conjugated linoleic acid
KW - Phosphatidylcholine
KW - Phospholipase A
KW - Structured phospholipids
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U2 - 10.3989/gya.045211
DO - 10.3989/gya.045211
M3 - Article
AN - SCOPUS:84856270519
VL - 63
SP - 44
EP - 52
JO - Grasas y Aceites
JF - Grasas y Aceites
SN - 0017-3495
IS - 1
ER -