Use of immobilized phospholipase A1-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content

R. Baeza-Jiménez; J. González-Rodríguez; In-Hwan Kim; H. S. García; C. Otero

doi:10.3989/gya.045211

Use of immobilized phospholipase A₁-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content

R. Baeza-Jiménez, J. González-Rodríguez, In-Hwan Kim, H. S. García, C. Otero

Department of Public Health Sciences

Research output: Contribution to journal › Article

14 Citations (Scopus)

Abstract

Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A₁ (PLA₁) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.

Original language	English
Pages (from-to)	44-52
Number of pages	9
Journal	Grasas y Aceites
Volume	63
Issue number	1
DOIs	https://doi.org/10.3989/gya.045211
Publication status	Published - 2012 Jan 1

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Keywords

Acidolysis
Conjugated linoleic acid
Phosphatidylcholine
Phospholipase A
Structured phospholipids

ASJC Scopus subject areas

Food Science
Organic Chemistry

Cite this

Baeza-Jiménez, R., González-Rodríguez, J., Kim, I-H., García, H. S., & Otero, C. (2012). Use of immobilized phospholipase A₁-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content. Grasas y Aceites, 63(1), 44-52. https://doi.org/10.3989/gya.045211

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AU - Otero, C.

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Access to Document

10.3989/gya.045211