Abstract
Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A1 (PLA1) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.
Original language | English |
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Pages (from-to) | 44-52 |
Number of pages | 9 |
Journal | Grasas y Aceites |
Volume | 63 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2012 Jan |
Keywords
- Acidolysis
- Conjugated linoleic acid
- Phosphatidylcholine
- Phospholipase A
- Structured phospholipids
ASJC Scopus subject areas
- Food Science
- Organic Chemistry