Abstract
Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A1 (PLA1) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.
| Original language | English |
|---|---|
| Pages (from-to) | 44-52 |
| Number of pages | 9 |
| Journal | Grasas y Aceites |
| Volume | 63 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2012 Jan 1 |
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Keywords
- Acidolysis
- Conjugated linoleic acid
- Phosphatidylcholine
- Phospholipase A
- Structured phospholipids
ASJC Scopus subject areas
- Food Science
- Organic Chemistry
Cite this
Use of immobilized phospholipase A1-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content. / Baeza-Jiménez, R.; González-Rodríguez, J.; Kim, In-Hwan; García, H. S.; Otero, C.
In: Grasas y Aceites, Vol. 63, No. 1, 01.01.2012, p. 44-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of immobilized phospholipase A1-catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content
AU - Baeza-Jiménez, R.
AU - González-Rodríguez, J.
AU - Kim, In-Hwan
AU - García, H. S.
AU - Otero, C.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A1 (PLA1) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.
AB - Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A1 (PLA1) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 °C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 °C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.
KW - Acidolysis
KW - Conjugated linoleic acid
KW - Phosphatidylcholine
KW - Phospholipase A
KW - Structured phospholipids
UR - http://www.scopus.com/inward/record.url?scp=84856270519&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856270519&partnerID=8YFLogxK
U2 - 10.3989/gya.045211
DO - 10.3989/gya.045211
M3 - Article
AN - SCOPUS:84856270519
VL - 63
SP - 44
EP - 52
JO - Grasas y Aceites
JF - Grasas y Aceites
SN - 0017-3495
IS - 1
ER -