Use of PCR-array to profile expressed genes in human keratinocyte hacat cells after exposure to Quantum Dots

Seung Ho Lee, Hee Ra Lee, Yu Ri Kim, Sang Hui Seo, In Kyoung Kim, Yoon Hee Park, Sang Hoon Jeong, Jong Pil Youn, Seung Yong Hwang, Sang Wook Son, Meyoung Kon Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Quantum dots (QDs) nanoparticles have potential applications in biomedical research, as imaging and diagnostic agents, because of their fluorescent property. Despite the widespread use of nanoparticles, there are many unknowns in understanding on their nanotoxicities and mechanisms. This study evaluated the effects of Quantum Dots (QDs) on gene expression profiles of human keratinocyte HaCaT cells. Total RNA was prepared from the exposure groups to QDs with diverse physicochemical properties (565-PEG-amine, 565-carboxylic acid, 655-carboxylic acid) in various concentrations, and real-time RT-PCR analysis was performed using human oxidative stress and antioxidant defense array. Our study indicated that antioxidant defense and oxidative stress category, such as nitric oxide synthase 2A (NOS2A), dual specificity phosphatase 1 (DUSP1), dual oxidase (DUOX), 24-dehydrocholesterol reductase (DHCR24), peroxidase in homolog (Drosophila)-like (PXDNL), scavenger receptor class A (SCARA3) and thyroid peroxidase (TPO), were represented by all three QDs exposure groups. Therefore, the up-regulation of these enzymes by QDs could increase the production of reactive oxygen species (ROS). The results of our study showed that QDs contained a potential to produce ROS via metabolic pathways, inducing oxidative stress. The results supported that the mechanism of nanotoxicity could be correlated with active oxygen production, oxidative stress, apoptosis, and antioxidant defense mechanisms.

Original languageEnglish
Pages (from-to)162-167
Number of pages6
JournalToxicology and Environmental Health Sciences
Volume2
Issue number3
DOIs
Publication statusPublished - 2010

Keywords

  • Antioxidant defense
  • Keratinocyte
  • Nanoparticles
  • PCR Array
  • Quantum dots
  • Reactive oxygen species

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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